Use of routine histopathology and factor VIII-related antigen/von Willebrand factor immunohistochemistry to differentiate primary hemangiosarcoma of bone from telangiectatic osteosarcoma in 54 dogs

Michelle Giuffrida, N. J. Bacon, D. A. Kamstock

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Hemangiosarcoma (HSA) of bone and telangiectatic osteosarcoma (tOSA) can appear similar histologically, but differ in histogenesis (malignant endothelial cells versus osteoblasts), and may warrant different treatments. Immunohistochemistry (IHC) for endothelial cell marker factor VIII-related antigen/von Willebrand factor (FVIII-RAg/vWF) is a well-documented ancillary test to confirm HSA diagnoses in soft tissues, but its use in osseous HSA is rarely described. Archived samples of 54 primary appendicular bone tumours previously diagnosed as HSA or tOSA were evaluated using combination routine histopathology (RHP) and IHC. Approximately 20% of tumours were reclassified on the basis of FVIII-RAg/vWF immunoreactivity, typically from an original diagnosis of tOSA to a reclassified diagnosis of HSA. No sample with tumour osteoid clearly identified on RHP was immunopositive for FVIII-RAg/vWF. RHP alone was specific but not sensitive for diagnosis of HSA, compared with combination RHP and IHC. Routine histopathological evaluation in combination with FVIII-RAg/vWF IHC can help differentiate canine primary appendicular HSA from tOSA.

Original languageEnglish (US)
Pages (from-to)1232-1239
Number of pages8
JournalVeterinary and Comparative Oncology
Volume15
Issue number4
DOIs
StatePublished - Dec 1 2017

Keywords

  • diagnostic tests and procedures
  • dogs
  • factor VIII-related antigen
  • hemangiosarcoma
  • immunohistochemistry
  • osteosarcoma

ASJC Scopus subject areas

  • veterinary(all)

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