Use of nuclear receptor luciferase-based bioassays to detect endocrine active chemicals in a biosolids-biochar amended soil

Carolyn G. Anderson, Geetika Joshi, Daniel A. Bair, Charlotte Oriol, Guochun He, Sanjai J. Parikh, Michael S. Denison, Kate M. Scow

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Biosolids are a potentially valuable source of carbon and nutrients for agricultural soils; however, potential unintended impacts on human health and the environment must be considered. Virtually all biosolids contain trace amounts endocrine-disrupting chemicals derived from human use of pharmaceuticals and personal care products (PPCPs). One potential way to reduce the bioavailability of PPCPs is to co-apply biosolids with biochar to soil, because biochar's chemical (e.g., aromaticity) and physical properties (e.g., surface area) give it a high affinity to bind many organic chemicals in the environment. We developed a soil-specific extraction method and utilized a luciferase-based bioassay (CALUX) to detect endocrine active chemicals in a biosolids-biochar co-amendment soil greenhouse study. Both biochar (walnut shell, 900 °C) and biosolids had positive impacts on carrot and lettuce biomass accumulation over our study period. However, the walnut shell biochar stimulated aryl hydrocarbon receptor activity, suggesting the presence of potential endocrine active chemicals in the biochar. Since the biochar rate tested (100 t ha−1) is above the average agronomic rate (10–20 t ha−1), endocrine effects would not be expected in most environmental applications. The effect of high temperature biochars on endocrine system pathways must be explored further, using both quantitative analytical tools to identify potential endocrine active chemicals and highly sensitive bioanalytical assays such as CALUX to measure the resulting biological activity of such compounds.

Original languageEnglish (US)
Pages (from-to)160-167
Number of pages8
JournalChemosphere
Volume181
DOIs
StatePublished - Aug 1 2017

Fingerprint

Biosolids
Bioassay
biosolid
Cytoplasmic and Nuclear Receptors
Luciferases
Biological Assay
bioassay
Soil
Soils
soil
Drug products
Juglans
Pharmaceutical Services
shell
endocrine system
soil amendment
Greenhouses
Organic chemicals
Bioactivity
extraction method

Keywords

  • Biochar
  • Biosolids
  • CALUX bioassay
  • Endocrine disruption
  • Land application

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry

Cite this

Use of nuclear receptor luciferase-based bioassays to detect endocrine active chemicals in a biosolids-biochar amended soil. / Anderson, Carolyn G.; Joshi, Geetika; Bair, Daniel A.; Oriol, Charlotte; He, Guochun; Parikh, Sanjai J.; Denison, Michael S.; Scow, Kate M.

In: Chemosphere, Vol. 181, 01.08.2017, p. 160-167.

Research output: Contribution to journalArticle

Anderson, Carolyn G. ; Joshi, Geetika ; Bair, Daniel A. ; Oriol, Charlotte ; He, Guochun ; Parikh, Sanjai J. ; Denison, Michael S. ; Scow, Kate M. / Use of nuclear receptor luciferase-based bioassays to detect endocrine active chemicals in a biosolids-biochar amended soil. In: Chemosphere. 2017 ; Vol. 181. pp. 160-167.
@article{4c66a68f08f14f87a4c4cc4d8d3c4fb0,
title = "Use of nuclear receptor luciferase-based bioassays to detect endocrine active chemicals in a biosolids-biochar amended soil",
abstract = "Biosolids are a potentially valuable source of carbon and nutrients for agricultural soils; however, potential unintended impacts on human health and the environment must be considered. Virtually all biosolids contain trace amounts endocrine-disrupting chemicals derived from human use of pharmaceuticals and personal care products (PPCPs). One potential way to reduce the bioavailability of PPCPs is to co-apply biosolids with biochar to soil, because biochar's chemical (e.g., aromaticity) and physical properties (e.g., surface area) give it a high affinity to bind many organic chemicals in the environment. We developed a soil-specific extraction method and utilized a luciferase-based bioassay (CALUX) to detect endocrine active chemicals in a biosolids-biochar co-amendment soil greenhouse study. Both biochar (walnut shell, 900 °C) and biosolids had positive impacts on carrot and lettuce biomass accumulation over our study period. However, the walnut shell biochar stimulated aryl hydrocarbon receptor activity, suggesting the presence of potential endocrine active chemicals in the biochar. Since the biochar rate tested (100 t ha−1) is above the average agronomic rate (10–20 t ha−1), endocrine effects would not be expected in most environmental applications. The effect of high temperature biochars on endocrine system pathways must be explored further, using both quantitative analytical tools to identify potential endocrine active chemicals and highly sensitive bioanalytical assays such as CALUX to measure the resulting biological activity of such compounds.",
keywords = "Biochar, Biosolids, CALUX bioassay, Endocrine disruption, Land application",
author = "Anderson, {Carolyn G.} and Geetika Joshi and Bair, {Daniel A.} and Charlotte Oriol and Guochun He and Parikh, {Sanjai J.} and Denison, {Michael S.} and Scow, {Kate M.}",
year = "2017",
month = "8",
day = "1",
doi = "10.1016/j.chemosphere.2017.04.035",
language = "English (US)",
volume = "181",
pages = "160--167",
journal = "Chemosphere",
issn = "0045-6535",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Use of nuclear receptor luciferase-based bioassays to detect endocrine active chemicals in a biosolids-biochar amended soil

AU - Anderson, Carolyn G.

AU - Joshi, Geetika

AU - Bair, Daniel A.

AU - Oriol, Charlotte

AU - He, Guochun

AU - Parikh, Sanjai J.

AU - Denison, Michael S.

AU - Scow, Kate M.

PY - 2017/8/1

Y1 - 2017/8/1

N2 - Biosolids are a potentially valuable source of carbon and nutrients for agricultural soils; however, potential unintended impacts on human health and the environment must be considered. Virtually all biosolids contain trace amounts endocrine-disrupting chemicals derived from human use of pharmaceuticals and personal care products (PPCPs). One potential way to reduce the bioavailability of PPCPs is to co-apply biosolids with biochar to soil, because biochar's chemical (e.g., aromaticity) and physical properties (e.g., surface area) give it a high affinity to bind many organic chemicals in the environment. We developed a soil-specific extraction method and utilized a luciferase-based bioassay (CALUX) to detect endocrine active chemicals in a biosolids-biochar co-amendment soil greenhouse study. Both biochar (walnut shell, 900 °C) and biosolids had positive impacts on carrot and lettuce biomass accumulation over our study period. However, the walnut shell biochar stimulated aryl hydrocarbon receptor activity, suggesting the presence of potential endocrine active chemicals in the biochar. Since the biochar rate tested (100 t ha−1) is above the average agronomic rate (10–20 t ha−1), endocrine effects would not be expected in most environmental applications. The effect of high temperature biochars on endocrine system pathways must be explored further, using both quantitative analytical tools to identify potential endocrine active chemicals and highly sensitive bioanalytical assays such as CALUX to measure the resulting biological activity of such compounds.

AB - Biosolids are a potentially valuable source of carbon and nutrients for agricultural soils; however, potential unintended impacts on human health and the environment must be considered. Virtually all biosolids contain trace amounts endocrine-disrupting chemicals derived from human use of pharmaceuticals and personal care products (PPCPs). One potential way to reduce the bioavailability of PPCPs is to co-apply biosolids with biochar to soil, because biochar's chemical (e.g., aromaticity) and physical properties (e.g., surface area) give it a high affinity to bind many organic chemicals in the environment. We developed a soil-specific extraction method and utilized a luciferase-based bioassay (CALUX) to detect endocrine active chemicals in a biosolids-biochar co-amendment soil greenhouse study. Both biochar (walnut shell, 900 °C) and biosolids had positive impacts on carrot and lettuce biomass accumulation over our study period. However, the walnut shell biochar stimulated aryl hydrocarbon receptor activity, suggesting the presence of potential endocrine active chemicals in the biochar. Since the biochar rate tested (100 t ha−1) is above the average agronomic rate (10–20 t ha−1), endocrine effects would not be expected in most environmental applications. The effect of high temperature biochars on endocrine system pathways must be explored further, using both quantitative analytical tools to identify potential endocrine active chemicals and highly sensitive bioanalytical assays such as CALUX to measure the resulting biological activity of such compounds.

KW - Biochar

KW - Biosolids

KW - CALUX bioassay

KW - Endocrine disruption

KW - Land application

UR - http://www.scopus.com/inward/record.url?scp=85018474521&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85018474521&partnerID=8YFLogxK

U2 - 10.1016/j.chemosphere.2017.04.035

DO - 10.1016/j.chemosphere.2017.04.035

M3 - Article

C2 - 28437741

AN - SCOPUS:85018474521

VL - 181

SP - 160

EP - 167

JO - Chemosphere

JF - Chemosphere

SN - 0045-6535

ER -