Use of multigeneration-family molecular dog leukocyte antigen typing to select a hematopoietic cell transplant donor for a dog with T-cell lymphoma

Marilena Lupu, Edmund W. Sullivan, Theresa E. Westfall, Marie Térèse Little, Benjamin J. Weigler, Peter F Moore, Patrice A. Stroup, Eustacia Zellmer, Christian Kuhr, Rainer Storb

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Case Description - A 7-year-old Golden Retriever was examined because of anorexia, lethargy, vomiting, and gradual weight loss. Clinical Findings - Splenomegaly, pancytopenia, high serum calcium concentration, and high alkaline phosphatase activity were detected. Magnetic resonance imaging revealed an enlarged mesenteric lymph node and increased signals from the bone marrow of the ilium and vertebral bodies. Histologic examination and immunophenotyping of biopsy specimens confirmed a stage V (b)T-cell malignant lymphoma. Treatment and Outcome - Clinical remission was attained by use of 2 chemotherapy cycles, followed by an allogeneic hematopoietic cell transplant performed at 18 weeks after diagnosis. A donor was identified by molecular dog leukocyte antigen typing methods. The patient was conditioned with 2 fractions of 4 Gy total body irradiation delivered 3 hours apart at 7 cGy/min, followed by an IV infusion of recombinant canine granulocyte colony-stimulating factor mobilized leukapheresis product and postgrafting immunosuppression with cyclosporine. Chimerism analyses revealed full donor engraftment that has been maintained for at least 58 weeks after transplant. Remission has been confirmed by normal results of serum thymidine kinase assays and the absence of peripheral blood clonal T-cell receptor gene rearrangements. Clinical Relevance - Systemic chemotherapy induces remissions; however, most dogs succumb to disease recurrence because of multidrug resistance. Outcome of allogeneic hematopoietic cell transplantation in dogs can be excellent because of improved donor-recipient selection by use of molecular dog leukocyte antigen typing, compared with early attempts, and better prevention of graft versus host disease, better supportive care, and substitution of peripheral blood mononuclear cells for bone marrow.

Original languageEnglish (US)
Pages (from-to)728-732
Number of pages5
JournalJournal of the American Veterinary Medical Association
Volume228
Issue number5
DOIs
StatePublished - Mar 1 2006

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T-Cell Lymphoma
HLA Antigens
lymphoma
leukocytes
T-lymphocytes
Tissue Donors
Dogs
antigens
Transplants
remission
dogs
blood serum
Bone Marrow
bone marrow
T-Lymphocyte Gene Rearrangement
drug therapy
cells
Ilium
Leukapheresis
T-Cell Receptor Genes

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Use of multigeneration-family molecular dog leukocyte antigen typing to select a hematopoietic cell transplant donor for a dog with T-cell lymphoma. / Lupu, Marilena; Sullivan, Edmund W.; Westfall, Theresa E.; Little, Marie Térèse; Weigler, Benjamin J.; Moore, Peter F; Stroup, Patrice A.; Zellmer, Eustacia; Kuhr, Christian; Storb, Rainer.

In: Journal of the American Veterinary Medical Association, Vol. 228, No. 5, 01.03.2006, p. 728-732.

Research output: Contribution to journalArticle

Lupu, Marilena ; Sullivan, Edmund W. ; Westfall, Theresa E. ; Little, Marie Térèse ; Weigler, Benjamin J. ; Moore, Peter F ; Stroup, Patrice A. ; Zellmer, Eustacia ; Kuhr, Christian ; Storb, Rainer. / Use of multigeneration-family molecular dog leukocyte antigen typing to select a hematopoietic cell transplant donor for a dog with T-cell lymphoma. In: Journal of the American Veterinary Medical Association. 2006 ; Vol. 228, No. 5. pp. 728-732.
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