Use of monoclonal antibodies for detection of infectious pancreatic necrosis virus by the enzyme-linked immunosorbent assay (ELISA)

J. Domínguez, Ronald Hedrick, J. M. Sánchez-Vizcaino

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

An enzyme-linked immunosorbent assay (ELISA) has been developed for identification of the Sp strain of infectious pancreatic necrosis virus (IPNV). This assay employed 2 monoclonal antibodies directed against 2 non-overlapping epitopes of the minor structural viral protein. It was used to demonstrate the virus antigen in cell cultures and was able to detect 10 ng ml- 1 of purified virus or 104 TCID50 ml- 1 in tissue culture fluid. The assay could be reduced to one step and be performed within 90 min with good sensitivity. No antigenic variation, affecting the epitopes recognized by these monoclonal antibodies, was observed on 17 IPNV isolates of Sp serotype tested.

Original languageEnglish (US)
Pages (from-to)506-510
Number of pages5
JournalPhytopathology
Volume79
Issue number5
DOIs
StatePublished - Jan 1 1989

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Infectious pancreatic necrosis virus
antibody detection
epitopes
monoclonal antibodies
enzyme-linked immunosorbent assay
antigenic variation
viruses
viral proteins
structural proteins
assays
tissue culture
serotypes
cell culture
antigens
fluids

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Use of monoclonal antibodies for detection of infectious pancreatic necrosis virus by the enzyme-linked immunosorbent assay (ELISA). / Domínguez, J.; Hedrick, Ronald; Sánchez-Vizcaino, J. M.

In: Phytopathology, Vol. 79, No. 5, 01.01.1989, p. 506-510.

Research output: Contribution to journalArticle

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