Use of high-performance liquid chromatographic fractionation of large RNA molecules in the assay of group I intron ribozyme activity

D. E. Georgopoulos, Michael J Leibowitz

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Ion-pair reversed-phase high-performance liquid chromatography (HPLC), which has previously been used to fractionate double-stranded DNA molecules, can be applied to single-stranded RNA molecules in the size range of 200-1000 nucleotides. This procedure permits RNA molecules to be separated and recovered rapidly in liquid medium, thereby facilitating recovery. We have used this system to separate an in vitro transcription product containing a group I intron ribozyme from the intermediates and products of the splicing reaction, permitting rapid assay of ribozyme activity without the use of radioactivity.

Original languageEnglish (US)
Pages (from-to)109-114
Number of pages6
JournalJournal of Chromatography A
Volume868
Issue number1
DOIs
StatePublished - Jan 28 2000
Externally publishedYes

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Keywords

  • Ribozymes
  • RNA

ASJC Scopus subject areas

  • Analytical Chemistry

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