Use of high-performance liquid chromatographic fractionation of large RNA molecules in the assay of group I intron ribozyme activity

D. E. Georgopoulos, Michael J Leibowitz

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Ion-pair reversed-phase high-performance liquid chromatography (HPLC), which has previously been used to fractionate double-stranded DNA molecules, can be applied to single-stranded RNA molecules in the size range of 200-1000 nucleotides. This procedure permits RNA molecules to be separated and recovered rapidly in liquid medium, thereby facilitating recovery. We have used this system to separate an in vitro transcription product containing a group I intron ribozyme from the intermediates and products of the splicing reaction, permitting rapid assay of ribozyme activity without the use of radioactivity.

Original languageEnglish (US)
Pages (from-to)109-114
Number of pages6
JournalJournal of Chromatography A
Volume868
Issue number1
DOIs
StatePublished - Jan 28 2000
Externally publishedYes

Fingerprint

Catalytic RNA
Fractionation
Introns
Assays
RNA
Molecules
Liquids
Reverse-Phase Chromatography
Radioactivity
Nucleotides
High Pressure Liquid Chromatography
High performance liquid chromatography
Transcription
Ions
DNA
Recovery
GIR1 ribozyme
In Vitro Techniques

Keywords

  • Ribozymes
  • RNA

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Use of high-performance liquid chromatographic fractionation of large RNA molecules in the assay of group I intron ribozyme activity. / Georgopoulos, D. E.; Leibowitz, Michael J.

In: Journal of Chromatography A, Vol. 868, No. 1, 28.01.2000, p. 109-114.

Research output: Contribution to journalArticle

@article{cce270d6c550451da119c46b2ff0027f,
title = "Use of high-performance liquid chromatographic fractionation of large RNA molecules in the assay of group I intron ribozyme activity",
abstract = "Ion-pair reversed-phase high-performance liquid chromatography (HPLC), which has previously been used to fractionate double-stranded DNA molecules, can be applied to single-stranded RNA molecules in the size range of 200-1000 nucleotides. This procedure permits RNA molecules to be separated and recovered rapidly in liquid medium, thereby facilitating recovery. We have used this system to separate an in vitro transcription product containing a group I intron ribozyme from the intermediates and products of the splicing reaction, permitting rapid assay of ribozyme activity without the use of radioactivity.",
keywords = "Ribozymes, RNA",
author = "Georgopoulos, {D. E.} and Leibowitz, {Michael J}",
year = "2000",
month = "1",
day = "28",
doi = "10.1016/S0021-9673(99)01178-4",
language = "English (US)",
volume = "868",
pages = "109--114",
journal = "Journal of Chromatography",
issn = "0021-9673",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Use of high-performance liquid chromatographic fractionation of large RNA molecules in the assay of group I intron ribozyme activity

AU - Georgopoulos, D. E.

AU - Leibowitz, Michael J

PY - 2000/1/28

Y1 - 2000/1/28

N2 - Ion-pair reversed-phase high-performance liquid chromatography (HPLC), which has previously been used to fractionate double-stranded DNA molecules, can be applied to single-stranded RNA molecules in the size range of 200-1000 nucleotides. This procedure permits RNA molecules to be separated and recovered rapidly in liquid medium, thereby facilitating recovery. We have used this system to separate an in vitro transcription product containing a group I intron ribozyme from the intermediates and products of the splicing reaction, permitting rapid assay of ribozyme activity without the use of radioactivity.

AB - Ion-pair reversed-phase high-performance liquid chromatography (HPLC), which has previously been used to fractionate double-stranded DNA molecules, can be applied to single-stranded RNA molecules in the size range of 200-1000 nucleotides. This procedure permits RNA molecules to be separated and recovered rapidly in liquid medium, thereby facilitating recovery. We have used this system to separate an in vitro transcription product containing a group I intron ribozyme from the intermediates and products of the splicing reaction, permitting rapid assay of ribozyme activity without the use of radioactivity.

KW - Ribozymes

KW - RNA

UR - http://www.scopus.com/inward/record.url?scp=0033984653&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033984653&partnerID=8YFLogxK

U2 - 10.1016/S0021-9673(99)01178-4

DO - 10.1016/S0021-9673(99)01178-4

M3 - Article

C2 - 10677084

AN - SCOPUS:0033984653

VL - 868

SP - 109

EP - 114

JO - Journal of Chromatography

JF - Journal of Chromatography

SN - 0021-9673

IS - 1

ER -