Abstract
Ion-pair reversed-phase high-performance liquid chromatography (HPLC), which has previously been used to fractionate double-stranded DNA molecules, can be applied to single-stranded RNA molecules in the size range of 200-1000 nucleotides. This procedure permits RNA molecules to be separated and recovered rapidly in liquid medium, thereby facilitating recovery. We have used this system to separate an in vitro transcription product containing a group I intron ribozyme from the intermediates and products of the splicing reaction, permitting rapid assay of ribozyme activity without the use of radioactivity.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 109-114 |
| Number of pages | 6 |
| Journal | Journal of Chromatography A |
| Volume | 868 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 28 2000 |
| Externally published | Yes |
Fingerprint
Keywords
- Ribozymes
- RNA
ASJC Scopus subject areas
- Analytical Chemistry
Cite this
Use of high-performance liquid chromatographic fractionation of large RNA molecules in the assay of group I intron ribozyme activity. / Georgopoulos, D. E.; Leibowitz, Michael J.
In: Journal of Chromatography A, Vol. 868, No. 1, 28.01.2000, p. 109-114.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Use of high-performance liquid chromatographic fractionation of large RNA molecules in the assay of group I intron ribozyme activity
AU - Georgopoulos, D. E.
AU - Leibowitz, Michael J
PY - 2000/1/28
Y1 - 2000/1/28
N2 - Ion-pair reversed-phase high-performance liquid chromatography (HPLC), which has previously been used to fractionate double-stranded DNA molecules, can be applied to single-stranded RNA molecules in the size range of 200-1000 nucleotides. This procedure permits RNA molecules to be separated and recovered rapidly in liquid medium, thereby facilitating recovery. We have used this system to separate an in vitro transcription product containing a group I intron ribozyme from the intermediates and products of the splicing reaction, permitting rapid assay of ribozyme activity without the use of radioactivity.
AB - Ion-pair reversed-phase high-performance liquid chromatography (HPLC), which has previously been used to fractionate double-stranded DNA molecules, can be applied to single-stranded RNA molecules in the size range of 200-1000 nucleotides. This procedure permits RNA molecules to be separated and recovered rapidly in liquid medium, thereby facilitating recovery. We have used this system to separate an in vitro transcription product containing a group I intron ribozyme from the intermediates and products of the splicing reaction, permitting rapid assay of ribozyme activity without the use of radioactivity.
KW - Ribozymes
KW - RNA
UR - http://www.scopus.com/inward/record.url?scp=0033984653&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033984653&partnerID=8YFLogxK
U2 - 10.1016/S0021-9673(99)01178-4
DO - 10.1016/S0021-9673(99)01178-4
M3 - Article
C2 - 10677084
AN - SCOPUS:0033984653
VL - 868
SP - 109
EP - 114
JO - Journal of Chromatography
JF - Journal of Chromatography
SN - 0021-9673
IS - 1
ER -