Abstract
Objective - To determine whether a detergent can prevent most of the early effects of IV infusion with Escherichia coli endotoxin (<100 ng/kg of body weight) in horses: marked pulmonary hypertension, acute leukopenia, and fever. Animals - 8 healthy adult horses (4 male, 4 female), 415 to 615 kg. Design and Procedure - Control and detergent experiments were performed in each horse while it was awake but sedated. In control experiments, 10 to 100 ng of E coli endotoxin/kg was given. In detergent experiments, 100 mg of detergent/kg was given 1 hour before injecting endotoxin, similar to the control experiments. Results - In control experiments, pulmonary arterial pressure increased transiently over 40 minutes by 33 ± 8 mm of Hg (mean ± SD; P < 0.001), then returned to baseline. Circulating leukocytes decreased to 47 ± 19% (P < 0.02) of baseline by 1 hour after endotoxin, then increased above baseline by 6 hours. Rectal temperature increased by 0.7 ± 0.4 C (P < 0.01). In detergent experiments, the increase in pulmonary arterial pressure was much less than that in the control experiments (8 ± 7 mm of Hg; P < 0.001). Circulating leukocytes did not decrease, and the increase in rectal temperature after endotoxin was blocked. Conclusions - This attenuation of the response to endotoxin may occur because the normal steps in the response of pulmonary intravascular macrophages (ie, endocytosis of endotoxin and subsequent release of inflammatory mediators) are altered by the detergent. This low-technology, inexpensive, and safe treatment could be an important new clinical tool for veterinarians in combating endotoxemia.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1063-1066 |
| Number of pages | 4 |
| Journal | American Journal of Veterinary Research |
| Volume | 57 |
| Issue number | 7 |
| State | Published - Jul 1 1996 |
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ASJC Scopus subject areas
- veterinary(all)
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Use of detergent to prevent initial responses to endotoxin in horses. / Longworth, Kim E.; Smith, Barbara L.; Staub, Norman C.; Steffey, Eugene; Serikov, Vladimir B.
In: American Journal of Veterinary Research, Vol. 57, No. 7, 01.07.1996, p. 1063-1066.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Use of detergent to prevent initial responses to endotoxin in horses
AU - Longworth, Kim E.
AU - Smith, Barbara L.
AU - Staub, Norman C.
AU - Steffey, Eugene
AU - Serikov, Vladimir B.
PY - 1996/7/1
Y1 - 1996/7/1
N2 - Objective - To determine whether a detergent can prevent most of the early effects of IV infusion with Escherichia coli endotoxin (<100 ng/kg of body weight) in horses: marked pulmonary hypertension, acute leukopenia, and fever. Animals - 8 healthy adult horses (4 male, 4 female), 415 to 615 kg. Design and Procedure - Control and detergent experiments were performed in each horse while it was awake but sedated. In control experiments, 10 to 100 ng of E coli endotoxin/kg was given. In detergent experiments, 100 mg of detergent/kg was given 1 hour before injecting endotoxin, similar to the control experiments. Results - In control experiments, pulmonary arterial pressure increased transiently over 40 minutes by 33 ± 8 mm of Hg (mean ± SD; P < 0.001), then returned to baseline. Circulating leukocytes decreased to 47 ± 19% (P < 0.02) of baseline by 1 hour after endotoxin, then increased above baseline by 6 hours. Rectal temperature increased by 0.7 ± 0.4 C (P < 0.01). In detergent experiments, the increase in pulmonary arterial pressure was much less than that in the control experiments (8 ± 7 mm of Hg; P < 0.001). Circulating leukocytes did not decrease, and the increase in rectal temperature after endotoxin was blocked. Conclusions - This attenuation of the response to endotoxin may occur because the normal steps in the response of pulmonary intravascular macrophages (ie, endocytosis of endotoxin and subsequent release of inflammatory mediators) are altered by the detergent. This low-technology, inexpensive, and safe treatment could be an important new clinical tool for veterinarians in combating endotoxemia.
AB - Objective - To determine whether a detergent can prevent most of the early effects of IV infusion with Escherichia coli endotoxin (<100 ng/kg of body weight) in horses: marked pulmonary hypertension, acute leukopenia, and fever. Animals - 8 healthy adult horses (4 male, 4 female), 415 to 615 kg. Design and Procedure - Control and detergent experiments were performed in each horse while it was awake but sedated. In control experiments, 10 to 100 ng of E coli endotoxin/kg was given. In detergent experiments, 100 mg of detergent/kg was given 1 hour before injecting endotoxin, similar to the control experiments. Results - In control experiments, pulmonary arterial pressure increased transiently over 40 minutes by 33 ± 8 mm of Hg (mean ± SD; P < 0.001), then returned to baseline. Circulating leukocytes decreased to 47 ± 19% (P < 0.02) of baseline by 1 hour after endotoxin, then increased above baseline by 6 hours. Rectal temperature increased by 0.7 ± 0.4 C (P < 0.01). In detergent experiments, the increase in pulmonary arterial pressure was much less than that in the control experiments (8 ± 7 mm of Hg; P < 0.001). Circulating leukocytes did not decrease, and the increase in rectal temperature after endotoxin was blocked. Conclusions - This attenuation of the response to endotoxin may occur because the normal steps in the response of pulmonary intravascular macrophages (ie, endocytosis of endotoxin and subsequent release of inflammatory mediators) are altered by the detergent. This low-technology, inexpensive, and safe treatment could be an important new clinical tool for veterinarians in combating endotoxemia.
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UR - http://www.scopus.com/inward/citedby.url?scp=0030017565&partnerID=8YFLogxK
M3 - Article
C2 - 8807023
AN - SCOPUS:0030017565
VL - 57
SP - 1063
EP - 1066
JO - American Journal of Veterinary Research
JF - American Journal of Veterinary Research
SN - 0002-9645
IS - 7
ER -