Use of designer recombinant mitochondrial antigens in the diagnosis of primary biliary cirrhosis

Patrick S Leung, Takashi Iwayama, Thomas P Prindiville, David T. Chuang, Aftab A. Ansari, R. Max Wynn, Rollie Dickson, Ross Coppel, M. Eric Gershwin

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

The appearance of autoantibodies against mitochondria in patients with primary biliary cirrhosis has been known for more than 25 yr. In the past, based on the biochemical complexity of the mitochondrion and the use of crude extracts for immunodiagnosis, a degree of nonspecificity in assaying for antibodies to mitochondria has been present. This problem has been largely circumvented by the cloning of the mitochondrial antigens and the identification of the E2 subunits of the pyruvate dehydrogenase complex and the branched chain 2-oxo-acid dehydrogenase complex as the major and immunodominant autoantigens of primary biliary cirrhosis. More than 90% of patients with primary biliary cirrhosis have been shown to react with one or both of these enzymes using either recombinant antigen or purified native protein. Approximately 10% of patients recognize only E2 subunits of branched chain 2-oxo-acid dehydrogenase complex and not pyruvate dehydrogenase complex. Such patients would be missed by diagnostic assay that has a low sensitivity to antibodies against E2 subunits of branched chain 2-oxo-acid dehydrogenase complex. The use of recombinant and biochemically pure antigens has permitted structural and conformational analysis of epitope mapping. We have taken advantage of the antigenic mapping studies of both primary biliary cirrhosis and branched chain 2-oxo-acid dehydrogenase complex E2 subunits and designed a molecule that expresses the immunodominant epitopes of both. Using this dual-headed molecule that coexpresses the epitope of two different antigens, we report herein a sensitive and reproducible assay for antibodies to mitochondria in patients with primary biliary cirrhosis. We believe that this recombinant protein is the first example of the use of designer molecules for immunodiagnosis.

Original languageEnglish (US)
Pages (from-to)367-372
Number of pages6
JournalHepatology
Volume15
Issue number3
StatePublished - Mar 1992

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3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)
Biliary Liver Cirrhosis
Mitochondria
Antigens
Immunologic Tests
Antibodies
Dihydrolipoyllysine-Residue Acetyltransferase
Pyruvate Dehydrogenase Complex
Epitope Mapping
Immunodominant Epitopes
Autoantigens
Complex Mixtures
Recombinant Proteins
Autoantibodies
Organism Cloning
Epitopes
Enzymes
Proteins

ASJC Scopus subject areas

  • Hepatology

Cite this

Use of designer recombinant mitochondrial antigens in the diagnosis of primary biliary cirrhosis. / Leung, Patrick S; Iwayama, Takashi; Prindiville, Thomas P; Chuang, David T.; Ansari, Aftab A.; Wynn, R. Max; Dickson, Rollie; Coppel, Ross; Gershwin, M. Eric.

In: Hepatology, Vol. 15, No. 3, 03.1992, p. 367-372.

Research output: Contribution to journalArticle

Leung, PS, Iwayama, T, Prindiville, TP, Chuang, DT, Ansari, AA, Wynn, RM, Dickson, R, Coppel, R & Gershwin, ME 1992, 'Use of designer recombinant mitochondrial antigens in the diagnosis of primary biliary cirrhosis', Hepatology, vol. 15, no. 3, pp. 367-372.
Leung, Patrick S ; Iwayama, Takashi ; Prindiville, Thomas P ; Chuang, David T. ; Ansari, Aftab A. ; Wynn, R. Max ; Dickson, Rollie ; Coppel, Ross ; Gershwin, M. Eric. / Use of designer recombinant mitochondrial antigens in the diagnosis of primary biliary cirrhosis. In: Hepatology. 1992 ; Vol. 15, No. 3. pp. 367-372.
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