Use of cultured airway epithelial cells in studies of ion transport

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

In 1984, primary cultures of dog tracheal epithelium were shown to retain the ion transport processes of the original tissue. Since then, ion transport in both nasal and tracheal cell cultures has been studied with Ussing chambers, conventional and ion-sensitive microelectrodes, radiotracers, Cl-sensing fluorescent dyes, and patch clamping. Much of the resulting information could not have been obtained using the native tissues and has been especially useful in determining the ion transport defect(s) in cystic fibrosis. Recent improvements in growth conditions should make highly differentiated cell cultures routinely available, further increasing the usefulness of cultured cells for the study of ion transport by airway epithelium.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume258
Issue number2 2-1
StatePublished - 1990
Externally publishedYes

Keywords

  • chloride secretion
  • cystic fibrosis
  • ion-sensitive microelectrodes
  • patch clamping
  • Ussing chambers

ASJC Scopus subject areas

  • Cell Biology
  • Physiology
  • Pulmonary and Respiratory Medicine

Fingerprint Dive into the research topics of 'Use of cultured airway epithelial cells in studies of ion transport'. Together they form a unique fingerprint.

  • Cite this