Use of conventional and real-time polymerase chain reaction to determine the epidemiology of hemoplasma infections in anemic and nonanemic cats

Jane E Sykes, Nicole L. Drazenovich, Louise M. Ball, Christian M. Leutenegger

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Background: The goals of this study were to develop and apply conventional (c) and real-time TaqMan polymerase chain reaction (PCR) assays for Mycoplasma haemofelis (Mhf), 'Candidatus Mycoplasma haematoparvum' (Mhp), and 'Candidatus Mycoplasma haemominutum' (Mhm) to blood samples of cats to determine the epidemiology of these infections in cats. Hypothesis: Cats are infected with > 2 hemoplasma species, and organism load correlates with disease induced by these organisms. Animals: Blood samples from 263 anemic and nonanemic cats were used. Methods: A retrospective study was conducted. Results: Forty-seven (18%) samples were positive. Three samples (1%) yielded 170 base pair cPCR products, 1 of which was positive for Mhf using real-time PCR. Forty-four samples (17%) yielded 193 base pair cPCR products, 40 of which were positive for Mhm using real-time PCR. Organism loads ranged from 375 × 10 6/mL to 6.9 × 10 6/mL of blood. Sequencing of cPCR products from samples testing negative using real-time PCR identified 2 Mhp-like sequences, 1 Mhm-Yike sequence, and 1 sequence resembling 'Candidatus Mycoplasma turicensis.' Cats infected with Mhm were less likely to be anemic than uninfected cats. Older age, outdoor exposure, feline immunodeficiency virus (FIV) seropositivity, cutaneous squamous cell carcinoma (SCC), and stomatitis were associated with Mhm infection. Cats from the Sacramento Valley were more often infected with Mhm than cats from the San Francisco bay area. Conclusions and Clinical Importance: Cats may be infected with 4 hemoplasma species. The association between Mhm infection, FIV, and SCC may reflect outdoor roaming status of infected cats. The clustered distribution of infection suggests an arthropod vector in transmission.

Original languageEnglish (US)
Pages (from-to)685-693
Number of pages9
JournalJournal of Veterinary Internal Medicine
Volume21
Issue number4
DOIs
StatePublished - Jul 2007

Fingerprint

Mycoplasma
Mycoplasma haemominutum
epidemiology
Real-Time Polymerase Chain Reaction
quantitative polymerase chain reaction
Epidemiology
Cats
cats
Infection
infection
Mycoplasma haemofelis
Feline Immunodeficiency Virus
Mycoplasma Infections
Feline immunodeficiency virus
squamous cell carcinoma
sampling
Base Pairing
Squamous Cell Carcinoma
blood
organisms

Keywords

  • Cat
  • Divergence
  • Haemobartonella
  • Infectious
  • Quantitative

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Use of conventional and real-time polymerase chain reaction to determine the epidemiology of hemoplasma infections in anemic and nonanemic cats. / Sykes, Jane E; Drazenovich, Nicole L.; Ball, Louise M.; Leutenegger, Christian M.

In: Journal of Veterinary Internal Medicine, Vol. 21, No. 4, 07.2007, p. 685-693.

Research output: Contribution to journalArticle

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