Use of carbamylated hemoglobin concentration to differentiate acute from chronic renal failure in dogs

Shelly L. Vaden, Jody Gookin, Maureen Trogdon, Cathy E. Langston, Jay Levine, Larry D Cowgill

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


Objective - To determine usefulness of carbamylated hemoglobin (CarHb) concentration for differentiation of acute renal failure (ARF) from chronic renal failure (CRF) in dogs. Sample Population - Samples from dogs with ARF or CRF and from nonazotemic control dogs. Procedure - CarHb concentration was determined in heparinized blood samples by measuring the micrograms of valine hydantoin (VH) per gram of hemoglobin (Hb), using a high-performance liquid chromatography assay, in which carbamyl valine is converted to VH via acid hydrolysis. Results - CarHb concentration was significantly higher in dogs with ARF and CRF, compared with values in control dogs (ARF vs control, P < 0.05; CRF vs control, P < 0.001). Furthermore, CarHb concentration was significantly (P < 0.001) higher in dogs with CRF, compared with that in dogs with ARF. Carbamylated hemoglobin concentration did not correlate with serum urea nitrogen or creatinine concentration. Using a cutoff value of 100 μg of VH/g of Hb, the sensitivity and specificity of CarHb concentration for differentiating ARF from CRF was 96.1 and 84.2%, respectively. Conclusions - CarHb concentration was useful in the differentiation of ARF from CRF in the dogs of this study. Clinical Relevance - CarHb concentration may be used to increase the accuracy of identifying ARF, so that early, aggressive management can be instituted, thereby increasing the chance of recovery.

Original languageEnglish (US)
Pages (from-to)1193-1196
Number of pages4
JournalAmerican Journal of Veterinary Research
Issue number11
StatePublished - Nov 1997

ASJC Scopus subject areas

  • veterinary(all)


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