Abstract
Objective - To determine usefulness of carbamylated hemoglobin (CarHb) concentration for differentiation of acute renal failure (ARF) from chronic renal failure (CRF) in dogs. Sample Population - Samples from dogs with ARF or CRF and from nonazotemic control dogs. Procedure - CarHb concentration was determined in heparinized blood samples by measuring the micrograms of valine hydantoin (VH) per gram of hemoglobin (Hb), using a high-performance liquid chromatography assay, in which carbamyl valine is converted to VH via acid hydrolysis. Results - CarHb concentration was significantly higher in dogs with ARF and CRF, compared with values in control dogs (ARF vs control, P < 0.05; CRF vs control, P < 0.001). Furthermore, CarHb concentration was significantly (P < 0.001) higher in dogs with CRF, compared with that in dogs with ARF. Carbamylated hemoglobin concentration did not correlate with serum urea nitrogen or creatinine concentration. Using a cutoff value of 100 μg of VH/g of Hb, the sensitivity and specificity of CarHb concentration for differentiating ARF from CRF was 96.1 and 84.2%, respectively. Conclusions - CarHb concentration was useful in the differentiation of ARF from CRF in the dogs of this study. Clinical Relevance - CarHb concentration may be used to increase the accuracy of identifying ARF, so that early, aggressive management can be instituted, thereby increasing the chance of recovery.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1193-1196 |
| Number of pages | 4 |
| Journal | American Journal of Veterinary Research |
| Volume | 58 |
| Issue number | 11 |
| State | Published - Nov 1997 |
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Use of carbamylated hemoglobin concentration to differentiate acute from chronic renal failure in dogs. / Vaden, Shelly L.; Gookin, Jody; Trogdon, Maureen; Langston, Cathy E.; Levine, Jay; Cowgill, Larry D.
In: American Journal of Veterinary Research, Vol. 58, No. 11, 11.1997, p. 1193-1196.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Use of carbamylated hemoglobin concentration to differentiate acute from chronic renal failure in dogs
AU - Vaden, Shelly L.
AU - Gookin, Jody
AU - Trogdon, Maureen
AU - Langston, Cathy E.
AU - Levine, Jay
AU - Cowgill, Larry D
PY - 1997/11
Y1 - 1997/11
N2 - Objective - To determine usefulness of carbamylated hemoglobin (CarHb) concentration for differentiation of acute renal failure (ARF) from chronic renal failure (CRF) in dogs. Sample Population - Samples from dogs with ARF or CRF and from nonazotemic control dogs. Procedure - CarHb concentration was determined in heparinized blood samples by measuring the micrograms of valine hydantoin (VH) per gram of hemoglobin (Hb), using a high-performance liquid chromatography assay, in which carbamyl valine is converted to VH via acid hydrolysis. Results - CarHb concentration was significantly higher in dogs with ARF and CRF, compared with values in control dogs (ARF vs control, P < 0.05; CRF vs control, P < 0.001). Furthermore, CarHb concentration was significantly (P < 0.001) higher in dogs with CRF, compared with that in dogs with ARF. Carbamylated hemoglobin concentration did not correlate with serum urea nitrogen or creatinine concentration. Using a cutoff value of 100 μg of VH/g of Hb, the sensitivity and specificity of CarHb concentration for differentiating ARF from CRF was 96.1 and 84.2%, respectively. Conclusions - CarHb concentration was useful in the differentiation of ARF from CRF in the dogs of this study. Clinical Relevance - CarHb concentration may be used to increase the accuracy of identifying ARF, so that early, aggressive management can be instituted, thereby increasing the chance of recovery.
AB - Objective - To determine usefulness of carbamylated hemoglobin (CarHb) concentration for differentiation of acute renal failure (ARF) from chronic renal failure (CRF) in dogs. Sample Population - Samples from dogs with ARF or CRF and from nonazotemic control dogs. Procedure - CarHb concentration was determined in heparinized blood samples by measuring the micrograms of valine hydantoin (VH) per gram of hemoglobin (Hb), using a high-performance liquid chromatography assay, in which carbamyl valine is converted to VH via acid hydrolysis. Results - CarHb concentration was significantly higher in dogs with ARF and CRF, compared with values in control dogs (ARF vs control, P < 0.05; CRF vs control, P < 0.001). Furthermore, CarHb concentration was significantly (P < 0.001) higher in dogs with CRF, compared with that in dogs with ARF. Carbamylated hemoglobin concentration did not correlate with serum urea nitrogen or creatinine concentration. Using a cutoff value of 100 μg of VH/g of Hb, the sensitivity and specificity of CarHb concentration for differentiating ARF from CRF was 96.1 and 84.2%, respectively. Conclusions - CarHb concentration was useful in the differentiation of ARF from CRF in the dogs of this study. Clinical Relevance - CarHb concentration may be used to increase the accuracy of identifying ARF, so that early, aggressive management can be instituted, thereby increasing the chance of recovery.
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M3 - Article
VL - 58
SP - 1193
EP - 1196
JO - American Journal of Veterinary Research
JF - American Journal of Veterinary Research
SN - 0002-9645
IS - 11
ER -