Use of capillary electrophoresis (CE) to quantitate carbamylated hemoglobin in dogs with renal failure

R. Heiene, Philip R Vulliet, R. L. Williams, Larry D Cowgill

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Abstract

The aims of the study were (1) to evaluate capillary electrophoresis (CE) for the measurement of carbamylated (CarbHb) and glycated hemoglobin (GlycHb) in normal, diabetic and azotemic dogs and (2) to determine if CarbHb, as measured by CE, could serve as a marker for chronic exposure to elevated blood levels of urea. CarbHb was measured in 8 dogs with renal failure, 2 dogs with diabetes mellitus and 7 control dogs. Optimal analytical conditions for separation of CarbHb and other hemoglobin derivatives in canine blood were determined using a commercial analysis system developed for the detection of the glycohemoglobin Hb Ale in human blood. The relative content of hemoglobin derivatives in blood from 4 dogs with acute renal failure, 3 dogs with chronic renal failure, 2 dogs with diabetes mellitus, and one dog on long-term hemodialysis were compared to 7 dogs with no renal or endocrine diseases. Satisfactory resolution of hemoglobin derivatives was obtained to permit identification and quantitation of CarbHb as a percentage of total hemoglobin. GlycHb at normal or increased amounts did not interfere with CarbHb analysis. Dogs with chronic renal failure had considerably higher CarbHb peaks than dogs with acute renal failure, one dog on dialysis, or dogs without known renal disease. CarbHb can be readily quantified by capillary electrophoresis in dog blood. The relative ease of sample preparation and short time for measurement of carbamylated hemoglobin by this method will facilitate the evaluation of acute and chronic aJ.otemia in patients with renal disease. (Figure Presented).

Original languageEnglish (US)
JournalBlood
Volume96
Issue number11 PART II
StatePublished - 2000

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ASJC Scopus subject areas

  • Hematology

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