Use of avian bornavirus isolates to induce proventricular dilatation disease in conures

Patricia Gray, Sharman Hoppes, Paulette Suchodolski, Negin Mirhosseini, Susan Payne, Itamar Villanueva, H L Shivaprasad, Kirsi S. Honkavuori, Thomas Briese, Sanjay M. Reddy, Ian Tizard

Research output: Contribution to journalArticle

70 Scopus citations

Abstract

Avian bornavirus (ABV) is a newly discovered member of the family Bornaviridae that has been associated with the development of a lethal neurologic syndrome in birds, termed proventricular dilatation disease (PDD). We successfully isolated and characterized ABV from the brains of 8 birds with confirmed PDD. One isolate was passed 6 times in duck embryo fibroblasts, and the infected cells were then injected intramuscularly into 2 healthy Patagonian conures (Cyanoliseus patagonis). Clinical PDD developed in both birds by 66 days postinfection. PDD was confirmed by necropsy and histopathologic examination. Reverse transcription-PCR showed that the inoculated ABV was in the brains of the 2 infected birds. A control bird that received uninfected tissue culture cells remained healthy until it was euthanized at 77 days. Necropsy and histopathologic examinations showed no abnormalities; PCR did not indicate ABV in its brain tissues.

Original languageEnglish (US)
Pages (from-to)473-479
Number of pages7
JournalEmerging Infectious Diseases
Volume16
Issue number3
DOIs
StatePublished - Mar 2010

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases
  • Epidemiology

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    Gray, P., Hoppes, S., Suchodolski, P., Mirhosseini, N., Payne, S., Villanueva, I., Shivaprasad, H. L., Honkavuori, K. S., Briese, T., Reddy, S. M., & Tizard, I. (2010). Use of avian bornavirus isolates to induce proventricular dilatation disease in conures. Emerging Infectious Diseases, 16(3), 473-479. https://doi.org/10.3201/eid1603.091257