Abstract
Attenuated total reflection Fourier transform infrared spectroscopy was used to monitor the formation of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), DMPC: lactosylceramide, and DMPC: GD3 lipid bilayers onto a zinc selenide surface. Infrared absorption peak position, bandwidth, and intensity were all used to monitor the formation, acyl chain ordering, and chemical environment within each bilayer. The results from this study indicate that the addition of glycosphingolipids into a DMPC lipid bilayer introduces decreases in both, acyl chain ordering, and homogeneity within the bilayer. GD3:DMPC lipid bilayers possess lipid chain characteristics that are indiscernible from those present in the lactosylceramide:DMPC bilayer, while possessing different structural head groups, indicating that the head group has little influence on the underlying lipid structure. Differences in the phosphate hydration are, however, evident between the three types of bilayer, with phosphate hydration decreasing in the order LacCer:DMPC (1223.4cm -1)>DMPC only (1226cm -1)>GD3:DMPC (1229.6cm -1).
| Original language | English (US) |
|---|---|
| Pages (from-to) | 374-377 |
| Number of pages | 4 |
| Journal | Colloids and Surfaces B: Biointerfaces |
| Volume | 94 |
| DOIs | |
| State | Published - Jun 1 2012 |
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Keywords
- Attenuated total reflection
- DMPC
- Fourier transform infrared
- GD3
- Lactosylceramide
- Lipid bilayer
ASJC Scopus subject areas
- Biotechnology
- Colloid and Surface Chemistry
- Physical and Theoretical Chemistry
- Surfaces and Interfaces
Cite this
Use of attenuated total reflectance Fourier transform infrared spectroscopy to study lactosylceramide and GD3 DMPC bilayers. / Hernandez, Mateo R.; Towns, Elyse N.; Moore, Jessica; Lee, Hyeyoung; German, J. Bruce; Lebrilla, Carlito B; Parikh, Atul N.; Land, Donald P.
In: Colloids and Surfaces B: Biointerfaces, Vol. 94, 01.06.2012, p. 374-377.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Use of attenuated total reflectance Fourier transform infrared spectroscopy to study lactosylceramide and GD3 DMPC bilayers
AU - Hernandez, Mateo R.
AU - Towns, Elyse N.
AU - Moore, Jessica
AU - Lee, Hyeyoung
AU - German, J. Bruce
AU - Lebrilla, Carlito B
AU - Parikh, Atul N.
AU - Land, Donald P.
PY - 2012/6/1
Y1 - 2012/6/1
N2 - Attenuated total reflection Fourier transform infrared spectroscopy was used to monitor the formation of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), DMPC: lactosylceramide, and DMPC: GD3 lipid bilayers onto a zinc selenide surface. Infrared absorption peak position, bandwidth, and intensity were all used to monitor the formation, acyl chain ordering, and chemical environment within each bilayer. The results from this study indicate that the addition of glycosphingolipids into a DMPC lipid bilayer introduces decreases in both, acyl chain ordering, and homogeneity within the bilayer. GD3:DMPC lipid bilayers possess lipid chain characteristics that are indiscernible from those present in the lactosylceramide:DMPC bilayer, while possessing different structural head groups, indicating that the head group has little influence on the underlying lipid structure. Differences in the phosphate hydration are, however, evident between the three types of bilayer, with phosphate hydration decreasing in the order LacCer:DMPC (1223.4cm -1)>DMPC only (1226cm -1)>GD3:DMPC (1229.6cm -1).
AB - Attenuated total reflection Fourier transform infrared spectroscopy was used to monitor the formation of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), DMPC: lactosylceramide, and DMPC: GD3 lipid bilayers onto a zinc selenide surface. Infrared absorption peak position, bandwidth, and intensity were all used to monitor the formation, acyl chain ordering, and chemical environment within each bilayer. The results from this study indicate that the addition of glycosphingolipids into a DMPC lipid bilayer introduces decreases in both, acyl chain ordering, and homogeneity within the bilayer. GD3:DMPC lipid bilayers possess lipid chain characteristics that are indiscernible from those present in the lactosylceramide:DMPC bilayer, while possessing different structural head groups, indicating that the head group has little influence on the underlying lipid structure. Differences in the phosphate hydration are, however, evident between the three types of bilayer, with phosphate hydration decreasing in the order LacCer:DMPC (1223.4cm -1)>DMPC only (1226cm -1)>GD3:DMPC (1229.6cm -1).
KW - Attenuated total reflection
KW - DMPC
KW - Fourier transform infrared
KW - GD3
KW - Lactosylceramide
KW - Lipid bilayer
UR - http://www.scopus.com/inward/record.url?scp=84862815949&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84862815949&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfb.2012.01.034
DO - 10.1016/j.colsurfb.2012.01.034
M3 - Article
VL - 94
SP - 374
EP - 377
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
SN - 0927-7765
ER -