Use of attenuated total reflectance Fourier transform infrared spectroscopy to monitor the development of lipid aggregate structures

Mateo R. Hernandez, Elyse N. Towns, Terry C. Ng, Brian C. Walsh, Richard Osibanjo, Atul N. Parikh, Donald P. Land

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Attenuated total reflectance Fourier transform infrared spectroscopy is used to monitor the adsorption of 100 nm 1,2-dipalmitoyl-sn-glycero-3- phosphocholine (DPPC) phospholipid vesicles to the surfaces of Ge, electrolessly deposited Au, and a well formed self-assembled monolayer of 1-octadecanethiol. The interaction of DPPC vesicles in solution with these different surfaces yields distinctly different surface structures: intact DPPC vesicles on Ge, a supported phospholipid bilayer on an electrolessly deposited Au surface, and a phospholipid monolayer onto the hydrophobic self-assembled monolayer. IR peak position, bandwidth, and intensity are used to confirm structure formation and quantitation of the amount of lipid that desorbs during film formation.

Original languageEnglish (US)
Pages (from-to)2842-2846
Number of pages5
JournalApplied Optics
Volume51
Issue number15
DOIs
StatePublished - May 20 2012

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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