Use of an Isolated Intestinal Circuit to Evaluate the Effect of Ischemia and Reperfusion on Mucosal Permeability of the Equine Jejunum

Nicholas J. Vatistas, Jorge Nieto, Linda Van Hoogmoed, Ian Gardner, Jack R. Snyder

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Objective - To evaluate the efficacy of an isolated perfusion circuit and the effect of ischemiareperfusion on mucosal permeability of the jejunum. Study Design - In vitro study of intestinal mucosal permeability. Animals - Twelve healthy adult horses. Methods - A control segment of jejunum was placed in an isolated perfusion circuit for 240 minutes and mucosal permeability was measured. After detecting no deleterious effects of the isolated system on the control intestine, low flow ischemia was created in experimental segments for 20, 40, 60 and 90 minutes followed by 60 minutes of reperfusion and mucosal permeability was evaluated. At the completion of the studies, histologic evaluation was used to determine mucosal grades, surface area, and volume. Results - Control tissue was maintained in the isolated circuit for 240 minutes without effect on mucosal grade, surface area, or volume relative to intact tissue. After ischemia-reperfusion, mucosal grade increased, and volume and surface area decreased progressively with longer periods of ischemia. Mucosal clearance of albumin remained constant during 240 minutes of perfusion in control tissue and was elevated after ischemia-reperfusion. Conclusions - No deleterious changes were noted in jejunum perfused with this isolated circuit, whereas alterations in mucosal permeability were present after ischemia-reperfusion. Clinical Relevance - The isolated perfusion circuit successfully maintained an isolated segment of jejunum within physiologic limits, and can be used to evaluate the effects of injury and the efficacy of pharmaceuticals to attenuate these changes.

Original languageEnglish (US)
Pages (from-to)52-61
Number of pages10
JournalVeterinary Surgery
Volume32
Issue number1
DOIs
StatePublished - Jan 2004

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Jejunum
ischemia
jejunum
Horses
Reperfusion
Permeability
permeability
Ischemia
horses
Perfusion
surface area
in vitro studies
Intestines
albumins
Albumins
intestines
experimental design
drugs
Wounds and Injuries
Pharmaceutical Preparations

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Use of an Isolated Intestinal Circuit to Evaluate the Effect of Ischemia and Reperfusion on Mucosal Permeability of the Equine Jejunum. / Vatistas, Nicholas J.; Nieto, Jorge; Van Hoogmoed, Linda; Gardner, Ian; Snyder, Jack R.

In: Veterinary Surgery, Vol. 32, No. 1, 01.2004, p. 52-61.

Research output: Contribution to journalArticle

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abstract = "Objective - To evaluate the efficacy of an isolated perfusion circuit and the effect of ischemiareperfusion on mucosal permeability of the jejunum. Study Design - In vitro study of intestinal mucosal permeability. Animals - Twelve healthy adult horses. Methods - A control segment of jejunum was placed in an isolated perfusion circuit for 240 minutes and mucosal permeability was measured. After detecting no deleterious effects of the isolated system on the control intestine, low flow ischemia was created in experimental segments for 20, 40, 60 and 90 minutes followed by 60 minutes of reperfusion and mucosal permeability was evaluated. At the completion of the studies, histologic evaluation was used to determine mucosal grades, surface area, and volume. Results - Control tissue was maintained in the isolated circuit for 240 minutes without effect on mucosal grade, surface area, or volume relative to intact tissue. After ischemia-reperfusion, mucosal grade increased, and volume and surface area decreased progressively with longer periods of ischemia. Mucosal clearance of albumin remained constant during 240 minutes of perfusion in control tissue and was elevated after ischemia-reperfusion. Conclusions - No deleterious changes were noted in jejunum perfused with this isolated circuit, whereas alterations in mucosal permeability were present after ischemia-reperfusion. Clinical Relevance - The isolated perfusion circuit successfully maintained an isolated segment of jejunum within physiologic limits, and can be used to evaluate the effects of injury and the efficacy of pharmaceuticals to attenuate these changes.",
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