Use of an extracorporeal circuit to evaluate effects of ischemia and reperfusion of the equine large colon

Linda M. Van Hoogmoed, Jack R. Snyder, Jorge Nieto, Nicholas J. Vatistas, Faye A. Harmon

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Objective - To determine efficacy of an extracorporeal circuit to maintain a segment of equine large colon for 3.5 hours and to evaluate the effect of low arterial flow on histologic and metabolic variables. Sample Population - Segments of large colon from 15 healthy adult horses. Procedure - The pelvic flexure was surgically removed and maintained in an isolated circuit. In the control group, tissue was evaluated for 3.5 hours, whereas in the low-flow group, arterial flow was reduced to 20% of baseline for 40 minutes followed by 2 hours of reperfusion. Various metabolic and hemodynamic variables were evaluated at 30-minute intervals. Effects of nitric oxide (NO) and L-N-nitro-arginine-methyl-ester (L-NAME) on contractile activity were determined, and histomorphologic evaluation was performed at the completion of the study. Results - Low-flow ischemia with reperfusion caused significant histomorphologic differences, compared with the control group. In the low-flow group, significant differences included reduction in PaCO2, reduction in bicarbonate concentrations, increase in PaO2, and an increase in base deficit in arterial and venous blood samples. Other significant differences included increases in PCV, protein concentration, total WBC count, and albumin clearance for the low-flow group. Differences were not detected in inhibitory activity of the low-flow group relative to the control tissue with or without addition of NO and L-NAME. Conclusion - The extracorporeal circuit maintained a segment of equine intestine for 3.5 hours and can be used to simulate ischemic injury. The extracorporeal circuit provides the potential to investigate pharmaceutic agents that can minimize intestinal injury. (Am J Vet Res 2000;61:1042-1051).

Original languageEnglish (US)
Pages (from-to)1042-1051
Number of pages10
JournalAmerican Journal of Veterinary Research
Volume61
Issue number9
StatePublished - Sep 2000

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ischemia
Horses
Reperfusion
colon
Colon
Ischemia
horses
Nitric Oxide
Control Groups
Wounds and Injuries
Bicarbonates
Intestines
Albumins
nitric oxide
arginine
Hemodynamics
esters
Pharmaceutical Preparations
Population
hemodynamics

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Use of an extracorporeal circuit to evaluate effects of ischemia and reperfusion of the equine large colon. / Van Hoogmoed, Linda M.; Snyder, Jack R.; Nieto, Jorge; Vatistas, Nicholas J.; Harmon, Faye A.

In: American Journal of Veterinary Research, Vol. 61, No. 9, 09.2000, p. 1042-1051.

Research output: Contribution to journalArticle

Van Hoogmoed, Linda M. ; Snyder, Jack R. ; Nieto, Jorge ; Vatistas, Nicholas J. ; Harmon, Faye A. / Use of an extracorporeal circuit to evaluate effects of ischemia and reperfusion of the equine large colon. In: American Journal of Veterinary Research. 2000 ; Vol. 61, No. 9. pp. 1042-1051.
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abstract = "Objective - To determine efficacy of an extracorporeal circuit to maintain a segment of equine large colon for 3.5 hours and to evaluate the effect of low arterial flow on histologic and metabolic variables. Sample Population - Segments of large colon from 15 healthy adult horses. Procedure - The pelvic flexure was surgically removed and maintained in an isolated circuit. In the control group, tissue was evaluated for 3.5 hours, whereas in the low-flow group, arterial flow was reduced to 20{\%} of baseline for 40 minutes followed by 2 hours of reperfusion. Various metabolic and hemodynamic variables were evaluated at 30-minute intervals. Effects of nitric oxide (NO) and L-N-nitro-arginine-methyl-ester (L-NAME) on contractile activity were determined, and histomorphologic evaluation was performed at the completion of the study. Results - Low-flow ischemia with reperfusion caused significant histomorphologic differences, compared with the control group. In the low-flow group, significant differences included reduction in PaCO2, reduction in bicarbonate concentrations, increase in PaO2, and an increase in base deficit in arterial and venous blood samples. Other significant differences included increases in PCV, protein concentration, total WBC count, and albumin clearance for the low-flow group. Differences were not detected in inhibitory activity of the low-flow group relative to the control tissue with or without addition of NO and L-NAME. Conclusion - The extracorporeal circuit maintained a segment of equine intestine for 3.5 hours and can be used to simulate ischemic injury. The extracorporeal circuit provides the potential to investigate pharmaceutic agents that can minimize intestinal injury. (Am J Vet Res 2000;61:1042-1051).",
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