Use of an extracorporeal circuit to evaluate effects of intraluminal distention and decompression on the equine jejunum

Jorge Nieto, Linda M. Van Hoogmoed, Sharon Spier, Nicholas J. Vatistas, Jack R. Snyder, Brenna L. Timmerman

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Objective-To use an extracorporeal circuit to evaluative ate effects of intraluminal distention on the jejunum of healthy horses. Sample Population-2 jejunal segments from each of 5 horses. Procedure-Jejunal segments were harvested and maintained in an extracorporeal circuit. One segment was subjected to distention (intraluminal pressure, 25 cm H2O) followed by decompression, and 1 segment was maintained without distention. The influence of distention-decompression on vascular resistance was calculated. Mucosal permeability was evaluated by measuring the clearance of albumin from blood to lumen. After distention and decompression, tissue specimens were collected for histomorphologic evaluation. In addition, the contractile response of the circular smooth muscle layer was determined following incubation with 3 prokinetic agents. Results-Intestinal vascular resistance increased during intraluminal distention and returned to baseline values after decompression. Albumin clearance rate increased after distention, compared with baseline and control values. Histologic examination of the distended segments revealed grade-1 and -2 lesions of the mucosal villus. Edema and hemorrhage were evident in the submucosa and muscular layers. Mesothelial cell loss, edema, and hemorrhage were also evident in the serosa. Mucosal surface area and villus tip height decreased and submucosal volume increased in the distended tissue. Compared with responses in control specimens, distention decreased the contractile response induced by cisapride, erythromycin, and metoclopramide. Conclusions and Clinical Relevance-Intraluminal distention of the jejunum followed by decompression increased mucosal permeability and injury and decreased responses to prokinetic agents. Horses with intraluminal intestinal distention may have a decreased response to prokinetic agent.

Original languageEnglish (US)
Pages (from-to)267-275
Number of pages9
JournalAmerican Journal of Veterinary Research
Volume63
Issue number2
StatePublished - Feb 1 2002

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Jejunum
Decompression
jejunum
Horses
villi
horses
blood vessels
edema
hemorrhage
albumins
permeability
metoclopramide
serosa
Vascular Resistance
erythromycin
Albumins
Permeability
Edema
lesions (animal)
smooth muscle

ASJC Scopus subject areas

  • veterinary(all)

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Use of an extracorporeal circuit to evaluate effects of intraluminal distention and decompression on the equine jejunum. / Nieto, Jorge; Van Hoogmoed, Linda M.; Spier, Sharon; Vatistas, Nicholas J.; Snyder, Jack R.; Timmerman, Brenna L.

In: American Journal of Veterinary Research, Vol. 63, No. 2, 01.02.2002, p. 267-275.

Research output: Contribution to journalArticle

Nieto, Jorge ; Van Hoogmoed, Linda M. ; Spier, Sharon ; Vatistas, Nicholas J. ; Snyder, Jack R. ; Timmerman, Brenna L. / Use of an extracorporeal circuit to evaluate effects of intraluminal distention and decompression on the equine jejunum. In: American Journal of Veterinary Research. 2002 ; Vol. 63, No. 2. pp. 267-275.
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