Use of age and milk production data to improve the ability of enzyme-linked immunosorbent assay test results to predict Mycobacterium avium ssp. paratuberculosis fecal culture status

Roy D. Berghaus, Thomas B Farver, Randall J. Anderson, John M Adaska, Ian Gardner

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Cows from 2 California dairies were tested for paratuberculosis at the end of lactation by using fecal culture and a commercially available serum enzyme-linked immunosorbent assay (ELISA) test kit. Individual cow characteristics and production variables were evaluated along with ELISA testing results as predictors of fecal culture status. In multivariable logistic regression analysis, age and a herd-standardized version of 305-day mature equivalent (305 ME) milk production were significant predictors of fecal culture status after adjusting for herd, quarter of the study year, and ELISA sample-to-positive (S/P) ratio. The area under a nonparametric receiver operating characteristic curve was significantly greater for a multivariable model that included age and the level of milk production when compared with a model without these covariates. In conclusion, consideration of cow-level covariates was useful as an aid in predicting Mycobacterium avium ssp. paratuberculosis (MAP) fecal culture status. For a given ELISA S/P ratio, older cows and those with lower 305 ME milk production relative to other cows in the herd were significantly more likely to be shedding MAP in their feces at the end of lactation.

Original languageEnglish (US)
Pages (from-to)233-242
Number of pages10
JournalJournal of Veterinary Diagnostic Investigation
Volume18
Issue number3
StatePublished - May 2006

Fingerprint

Paratuberculosis
Mycobacterium avium
paratuberculosis
milk production
Milk
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
cows
Lactation
herds
testing
lactation
Feces
ROC Curve
analytical kits
Logistic Models
Regression Analysis
dairies
regression analysis
feces

Keywords

  • Dairy cattle
  • Fecal culture
  • Johne's disease
  • Mycobacterium paratuberculosis

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

Cite this

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title = "Use of age and milk production data to improve the ability of enzyme-linked immunosorbent assay test results to predict Mycobacterium avium ssp. paratuberculosis fecal culture status",
abstract = "Cows from 2 California dairies were tested for paratuberculosis at the end of lactation by using fecal culture and a commercially available serum enzyme-linked immunosorbent assay (ELISA) test kit. Individual cow characteristics and production variables were evaluated along with ELISA testing results as predictors of fecal culture status. In multivariable logistic regression analysis, age and a herd-standardized version of 305-day mature equivalent (305 ME) milk production were significant predictors of fecal culture status after adjusting for herd, quarter of the study year, and ELISA sample-to-positive (S/P) ratio. The area under a nonparametric receiver operating characteristic curve was significantly greater for a multivariable model that included age and the level of milk production when compared with a model without these covariates. In conclusion, consideration of cow-level covariates was useful as an aid in predicting Mycobacterium avium ssp. paratuberculosis (MAP) fecal culture status. For a given ELISA S/P ratio, older cows and those with lower 305 ME milk production relative to other cows in the herd were significantly more likely to be shedding MAP in their feces at the end of lactation.",
keywords = "Dairy cattle, Fecal culture, Johne's disease, Mycobacterium paratuberculosis",
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AU - Adaska, John M

AU - Gardner, Ian

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