Use of a rotating bioreactor toward tissue engineering the temporomandibular joint disc

Michael S. Detamore, Kymiacos A. Athanasiou

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

This objective of this study was to determine the effects of a rotating bioreactor in temporomandibular joint (TMJ) disc tissue engineering. Porcine TMJ disc cells were seeded at a density of 20 million cells/mL onto nonwoven poly(glycolic acid) (PGA) scaffolds in spinner flasks for 1 week and then cultured either under static conditions or in a rotating bioreactor for a period of 6 weeks. A series of analyses was performed, including mechanical testing, measurement of cellularity, quantification of matrix biosynthesis with a hydroxyproline assay and enzyme-linked immunosorbent assays, and observation of matrix distribution with immunohistochemistry. Between the bioreactor and static cultures, there were marked differences in gross appearance, histological structure, and distribution of collagen types I and II. Engineered constructs from the bioreactor contracted earlier and to a greater extent, resulting in a denser matrix and cell composition. In addition, immunostaining intensity was generally uniform in static constructs, in contrast to higher intensity around the periphery of bioreactor constructs. Moreover, bioreactor constructs had higher amounts of collagen II than did static constructs. However, differences in total matrix content and compressive stiffness were generally not significant. On the basis of the results of this study there is no clear benefit from use of the rotating bioreactor, although a sequence of static culture followed by rotating bioreactor culture may prove in the future to be more beneficial than either alone.

Original languageEnglish (US)
Pages (from-to)1188-1197
Number of pages10
JournalTissue Engineering
Volume11
Issue number7-8
DOIs
StatePublished - Jul 2005
Externally publishedYes

Fingerprint

Temporomandibular Joint Disc
Bioreactors
Tissue Engineering
Tissue engineering
glycolic acid
Collagen
Assays
Hydroxyproline
Prostaglandins A
Collagen Type II
Temporomandibular Joint
Mechanical testing
Biosynthesis
Collagen Type I
Scaffolds
Swine
Enzymes
Enzyme-Linked Immunosorbent Assay
Immunohistochemistry
Stiffness

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology
  • Biotechnology

Cite this

Use of a rotating bioreactor toward tissue engineering the temporomandibular joint disc. / Detamore, Michael S.; Athanasiou, Kymiacos A.

In: Tissue Engineering, Vol. 11, No. 7-8, 07.2005, p. 1188-1197.

Research output: Contribution to journalArticle

Detamore, Michael S. ; Athanasiou, Kymiacos A. / Use of a rotating bioreactor toward tissue engineering the temporomandibular joint disc. In: Tissue Engineering. 2005 ; Vol. 11, No. 7-8. pp. 1188-1197.
@article{6504ce61b307473589b5d50d169ee726,
title = "Use of a rotating bioreactor toward tissue engineering the temporomandibular joint disc",
abstract = "This objective of this study was to determine the effects of a rotating bioreactor in temporomandibular joint (TMJ) disc tissue engineering. Porcine TMJ disc cells were seeded at a density of 20 million cells/mL onto nonwoven poly(glycolic acid) (PGA) scaffolds in spinner flasks for 1 week and then cultured either under static conditions or in a rotating bioreactor for a period of 6 weeks. A series of analyses was performed, including mechanical testing, measurement of cellularity, quantification of matrix biosynthesis with a hydroxyproline assay and enzyme-linked immunosorbent assays, and observation of matrix distribution with immunohistochemistry. Between the bioreactor and static cultures, there were marked differences in gross appearance, histological structure, and distribution of collagen types I and II. Engineered constructs from the bioreactor contracted earlier and to a greater extent, resulting in a denser matrix and cell composition. In addition, immunostaining intensity was generally uniform in static constructs, in contrast to higher intensity around the periphery of bioreactor constructs. Moreover, bioreactor constructs had higher amounts of collagen II than did static constructs. However, differences in total matrix content and compressive stiffness were generally not significant. On the basis of the results of this study there is no clear benefit from use of the rotating bioreactor, although a sequence of static culture followed by rotating bioreactor culture may prove in the future to be more beneficial than either alone.",
author = "Detamore, {Michael S.} and Athanasiou, {Kymiacos A.}",
year = "2005",
month = "7",
doi = "10.1089/ten.2005.11.1188",
language = "English (US)",
volume = "11",
pages = "1188--1197",
journal = "Tissue Engineering",
issn = "1076-3279",
publisher = "Mary Ann Liebert Inc.",
number = "7-8",

}

TY - JOUR

T1 - Use of a rotating bioreactor toward tissue engineering the temporomandibular joint disc

AU - Detamore, Michael S.

AU - Athanasiou, Kymiacos A.

PY - 2005/7

Y1 - 2005/7

N2 - This objective of this study was to determine the effects of a rotating bioreactor in temporomandibular joint (TMJ) disc tissue engineering. Porcine TMJ disc cells were seeded at a density of 20 million cells/mL onto nonwoven poly(glycolic acid) (PGA) scaffolds in spinner flasks for 1 week and then cultured either under static conditions or in a rotating bioreactor for a period of 6 weeks. A series of analyses was performed, including mechanical testing, measurement of cellularity, quantification of matrix biosynthesis with a hydroxyproline assay and enzyme-linked immunosorbent assays, and observation of matrix distribution with immunohistochemistry. Between the bioreactor and static cultures, there were marked differences in gross appearance, histological structure, and distribution of collagen types I and II. Engineered constructs from the bioreactor contracted earlier and to a greater extent, resulting in a denser matrix and cell composition. In addition, immunostaining intensity was generally uniform in static constructs, in contrast to higher intensity around the periphery of bioreactor constructs. Moreover, bioreactor constructs had higher amounts of collagen II than did static constructs. However, differences in total matrix content and compressive stiffness were generally not significant. On the basis of the results of this study there is no clear benefit from use of the rotating bioreactor, although a sequence of static culture followed by rotating bioreactor culture may prove in the future to be more beneficial than either alone.

AB - This objective of this study was to determine the effects of a rotating bioreactor in temporomandibular joint (TMJ) disc tissue engineering. Porcine TMJ disc cells were seeded at a density of 20 million cells/mL onto nonwoven poly(glycolic acid) (PGA) scaffolds in spinner flasks for 1 week and then cultured either under static conditions or in a rotating bioreactor for a period of 6 weeks. A series of analyses was performed, including mechanical testing, measurement of cellularity, quantification of matrix biosynthesis with a hydroxyproline assay and enzyme-linked immunosorbent assays, and observation of matrix distribution with immunohistochemistry. Between the bioreactor and static cultures, there were marked differences in gross appearance, histological structure, and distribution of collagen types I and II. Engineered constructs from the bioreactor contracted earlier and to a greater extent, resulting in a denser matrix and cell composition. In addition, immunostaining intensity was generally uniform in static constructs, in contrast to higher intensity around the periphery of bioreactor constructs. Moreover, bioreactor constructs had higher amounts of collagen II than did static constructs. However, differences in total matrix content and compressive stiffness were generally not significant. On the basis of the results of this study there is no clear benefit from use of the rotating bioreactor, although a sequence of static culture followed by rotating bioreactor culture may prove in the future to be more beneficial than either alone.

UR - http://www.scopus.com/inward/record.url?scp=24944505565&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=24944505565&partnerID=8YFLogxK

U2 - 10.1089/ten.2005.11.1188

DO - 10.1089/ten.2005.11.1188

M3 - Article

C2 - 16144455

AN - SCOPUS:24944505565

VL - 11

SP - 1188

EP - 1197

JO - Tissue Engineering

JF - Tissue Engineering

SN - 1076-3279

IS - 7-8

ER -