Use of a recombinant envelope protein subunit antigen for specific serological diagnosis of West Nile virus infection

David W C Beasley, Michael R. Holbrook, Amelia P A Travassos Da Rosa, Lark L Schneider, Anne Sophie Carrara, Kathrine Phillippi-Falkenstein, Rudolf P. Bohm, Marion S. Ratterree, Kristy M. Lillibridge, George V. Ludwig, Jose Estrada-Franco, Scott C. Weaver, Robert B. Tesh, Robert E. Shope, Alan D T Barrett

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50 Scopus citations

Abstract

Serological diagnosis of West Nile virus (WNV) infection is complicated by extensive antigenic cross-reactivity with other closely related flaviviruses, such as St. Louis encephalitis virus. Here we describe a recombinant, bacterially expressed antigen equivalent to structural domain III of the WNV envelope protein that has allowed clear discrimination of antibody responses to WNV from those against other related flaviviruses in indirect enzyme-linked immunosorbent assays using standardized control antisera and field-collected samples.

Original languageEnglish (US)
Pages (from-to)2759-2765
Number of pages7
JournalJournal of Clinical Microbiology
Volume42
Issue number6
DOIs
StatePublished - Jun 2004
Externally publishedYes

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ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Beasley, D. W. C., Holbrook, M. R., Travassos Da Rosa, A. P. A., Schneider, L. L., Carrara, A. S., Phillippi-Falkenstein, K., Bohm, R. P., Ratterree, M. S., Lillibridge, K. M., Ludwig, G. V., Estrada-Franco, J., Weaver, S. C., Tesh, R. B., Shope, R. E., & Barrett, A. D. T. (2004). Use of a recombinant envelope protein subunit antigen for specific serological diagnosis of West Nile virus infection. Journal of Clinical Microbiology, 42(6), 2759-2765. https://doi.org/10.1128/JCM.42.6.2759-2765.2004