Urinary levels of insecticide metabolites and DNA damage in human sperm

John D. Meeker, Narendra P. Singh, Louise Ryan, Susan M. Duty, Dana B. Barr, Robert F. Herrick, Deborah H Bennett, Russ Hauser

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Background: Members of the general population are exposed to non-persistent insecticides at low levels. The present study explored whether environmental exposures to carbaryl and chlorpyrifos are associated with DNA damage in human sperm. Methods: Subjects (n = 260) were recruited through a Massachusetts infertility clinic. Individual exposures were measured as spot urinary metabolite concentrations of chlorpyrifos [3,5,6-trichloro-2-pyridinol (TCPY)] and carbaryl [1-naphthol (1N)], adjusted using specific gravity. Sperm DNA integrity was assessed by neutral comet assay and reported as comet extent, percentage DNA in comet tail (Tail%) and tail distributed moment (TDM). Results: A statistically significant increase in Tail% was found for an interquartile range (IQR) increase in both 1N [coefficient = 4.1; 95% confidence interval (CI) 1.9-6.3] and TCPY (2.8; 0.9-4.6), while a decrease in TDM was associated with IQR changes in 1N (-2.2; -4.9 to 0.5) and TCPY (-2.5; -4.7 to -0.2). A negative correlation between Tail% and TDM was present only when stratified by comet extent, suggesting that Tail% and TDM may measure different types of DNA damage within comet extent strata. Conclusions: Environmental exposure to carbaryl and chlorpyrifos may be associated with increased DNA damage in human sperm, as indicated by a change in comet assay parameters.

Original languageEnglish (US)
Pages (from-to)2573-2580
Number of pages8
JournalHuman Reproduction
Volume19
Issue number11
DOIs
StatePublished - Nov 2004
Externally publishedYes

Fingerprint

Insecticides
DNA Damage
Spermatozoa
Tail
Carbaryl
Chlorpyrifos
Comet Assay
Environmental Exposure
Specific Gravity
DNA
Infertility
Confidence Intervals
Population
3,5,6-trichloro-2-pyridinol
1-naphthol

Keywords

  • Comet assay
  • DNA damage
  • Exposure
  • Insecticides

ASJC Scopus subject areas

  • Physiology
  • Developmental Biology
  • Obstetrics and Gynecology
  • Reproductive Medicine

Cite this

Meeker, J. D., Singh, N. P., Ryan, L., Duty, S. M., Barr, D. B., Herrick, R. F., ... Hauser, R. (2004). Urinary levels of insecticide metabolites and DNA damage in human sperm. Human Reproduction, 19(11), 2573-2580. https://doi.org/10.1093/humrep/deh444

Urinary levels of insecticide metabolites and DNA damage in human sperm. / Meeker, John D.; Singh, Narendra P.; Ryan, Louise; Duty, Susan M.; Barr, Dana B.; Herrick, Robert F.; Bennett, Deborah H; Hauser, Russ.

In: Human Reproduction, Vol. 19, No. 11, 11.2004, p. 2573-2580.

Research output: Contribution to journalArticle

Meeker, JD, Singh, NP, Ryan, L, Duty, SM, Barr, DB, Herrick, RF, Bennett, DH & Hauser, R 2004, 'Urinary levels of insecticide metabolites and DNA damage in human sperm', Human Reproduction, vol. 19, no. 11, pp. 2573-2580. https://doi.org/10.1093/humrep/deh444
Meeker JD, Singh NP, Ryan L, Duty SM, Barr DB, Herrick RF et al. Urinary levels of insecticide metabolites and DNA damage in human sperm. Human Reproduction. 2004 Nov;19(11):2573-2580. https://doi.org/10.1093/humrep/deh444
Meeker, John D. ; Singh, Narendra P. ; Ryan, Louise ; Duty, Susan M. ; Barr, Dana B. ; Herrick, Robert F. ; Bennett, Deborah H ; Hauser, Russ. / Urinary levels of insecticide metabolites and DNA damage in human sperm. In: Human Reproduction. 2004 ; Vol. 19, No. 11. pp. 2573-2580.
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abstract = "Background: Members of the general population are exposed to non-persistent insecticides at low levels. The present study explored whether environmental exposures to carbaryl and chlorpyrifos are associated with DNA damage in human sperm. Methods: Subjects (n = 260) were recruited through a Massachusetts infertility clinic. Individual exposures were measured as spot urinary metabolite concentrations of chlorpyrifos [3,5,6-trichloro-2-pyridinol (TCPY)] and carbaryl [1-naphthol (1N)], adjusted using specific gravity. Sperm DNA integrity was assessed by neutral comet assay and reported as comet extent, percentage DNA in comet tail (Tail{\%}) and tail distributed moment (TDM). Results: A statistically significant increase in Tail{\%} was found for an interquartile range (IQR) increase in both 1N [coefficient = 4.1; 95{\%} confidence interval (CI) 1.9-6.3] and TCPY (2.8; 0.9-4.6), while a decrease in TDM was associated with IQR changes in 1N (-2.2; -4.9 to 0.5) and TCPY (-2.5; -4.7 to -0.2). A negative correlation between Tail{\%} and TDM was present only when stratified by comet extent, suggesting that Tail{\%} and TDM may measure different types of DNA damage within comet extent strata. Conclusions: Environmental exposure to carbaryl and chlorpyrifos may be associated with increased DNA damage in human sperm, as indicated by a change in comet assay parameters.",
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