Urinary and plasma estrogen conjugates, estradiol and estrone concentrations in nonpregnant and early pregnant mares

P. F. Daels, D. C. Ammon, G. H. Stabenfeldt, Irwin Liu, J. P. Hughes, B. L. Lasley

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

A direct radioimmunoassay for estrogen conjugates (EC) was applied to paired blood and urine samples collected from 20 mares and compared against estrone (E1) and estradiol-17β (E2) to monitor changes in estrogen production during ovulatory cycles and early pregnancy. Blood samples were taken daily from five mares through two consecutive ovulations and from six mares at 6-h intervals starting 48 hours prior to ovulation and continuing after ovulation had occurred. Blood samples were also collected daily or three times per week from conception until Day 60 of pregnancy in nine pregnant mares. The mean urinary EC, plasma EC and plasma E2 dynamics were parallel in nonpregnant mares, with a 3-fold increase in mean urinary EC concentrations from baseline to the ovulatory peak, a 1.8-fold increase in mean plasma EC concentrations and a 1.4-fold increase in mean plasma E2 concentrations. In early pregnancy, a two-fold increase in mean plasma E1 and EC concentrations occurred in concert with a five-fold rise in mean urinary EC concentrations, whereas plasma E2 did not change. Following hydrolysis and chromatographic separation, E1 and E2 were identified as the hydrolytic products in the urine of nonpregnant and pregnant mares; however, an unidentified estrogen was the major hydrolytic product in nonpregnant mares and pregnant mares prior to Day 38 of pregnancy. The increased resolution of the EC profiles compared with the profiles of other estrogen components indicates that the determination of EC in urine or plasma provides a useful alternative method for monitoring reproductive events in mares.

Original languageEnglish (US)
Pages (from-to)1001-1017
Number of pages17
JournalTheriogenology
Volume35
Issue number5
DOIs
StatePublished - Jan 1 1991

Fingerprint

conjugated estrogens
estrone
Estrone
mares
estradiol
Estradiol
Estrogens
pregnancy
estrogens
ovulation
urine
Ovulation
Pregnancy
blood
Urine
monitoring
radioimmunoassays
sampling
hydrolysis

Keywords

  • estrogen
  • mare
  • ovulation
  • pregnancy
  • urinary estrogen

ASJC Scopus subject areas

  • Small Animals
  • Food Animals
  • Animal Science and Zoology
  • Equine

Cite this

Urinary and plasma estrogen conjugates, estradiol and estrone concentrations in nonpregnant and early pregnant mares. / Daels, P. F.; Ammon, D. C.; Stabenfeldt, G. H.; Liu, Irwin; Hughes, J. P.; Lasley, B. L.

In: Theriogenology, Vol. 35, No. 5, 01.01.1991, p. 1001-1017.

Research output: Contribution to journalArticle

Daels, P. F. ; Ammon, D. C. ; Stabenfeldt, G. H. ; Liu, Irwin ; Hughes, J. P. ; Lasley, B. L. / Urinary and plasma estrogen conjugates, estradiol and estrone concentrations in nonpregnant and early pregnant mares. In: Theriogenology. 1991 ; Vol. 35, No. 5. pp. 1001-1017.
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