Uricase from fish liver: Isolation and some properties

J. E. Kinsella, B. German, J. Shetty

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

1. 1. The uricase (urate: oxygen oxidoreducase EC.1.7.3.3) activities in livers from rainbow trout, mackerel, lake trout, catfish, shark and tilapia were 1000, 1180, 920, 630, 490 and 420 units (in moles uric acid oxidized mg-1 protein min-1) per gram liver, respectively. 2. 2. The enzyme from lake trout was purified twenty fold by ammonium sulfate precipitation, protamine sulfate treatment and Sephacryl S-200 column chromatography. 3. 3. SDS-polyacrylamide gel-electrophoresis indicated an oligomeric enzyme containing subunits of 32,500 daltons. 4. 4. The pH optimum was 8.8 but the enzyme had a relatively broad pH activity profile between pH 7.0-9.5. 5. 5. Apparent Km and Vmax values of 80 μM and > 1000 was obtained for the trout liver enzyme.

Original languageEnglish (US)
Pages (from-to)621-624
Number of pages4
JournalComparative Biochemistry and Physiology -- Part B: Biochemistry and
Volume82
Issue number4
DOIs
StatePublished - 1985
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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