Uptake of tritiated nucleic acid precursors by Babesia bovis in vitro

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Uptake of tritiated nucleic acid precursors by Babesia bovis in vitro. International Journal for Parasitology 16: 263-268. The uptake of tritiated nucleic aid precursors in vitro by two Babesia bovis isolates, from Mexico and South Africa, was compared using a semiautomated microdilution method. The purine bases, adenine and hypoxanthine, and the purine nucleosides, adenosine and guanosine, were readily taken up into infected erythrocytes in cultures of both B. bovis isolates over two consecutive 24 h incubation periods. The uptake of adenosine and hypoxanthine was consistently greater than that of the other precursors. Tritiated uridine and cytidine appeared to be taken up into B. bovis infected erythrocytes, but thymidine was not. The ability to establish continuous cultures of B. bovis with blood from infected cattle which have less than a 1% parasitaemia, and to maintain dividing parasites that incorporate tritiated nucleotide precursors during incubation periods of 24-48 h, indicates that the methodology described could be utilized in immunological and biochemical studies on B. bovis, or to evaluate the babesicidal effect of chemotherapeutic agents.

Original languageEnglish (US)
Pages (from-to)263-268
Number of pages6
JournalInternational Journal for Parasitology
Volume16
Issue number3
DOIs
StatePublished - 1986
Externally publishedYes

Keywords

  • adenine
  • adenosine
  • Babesia bovis
  • cultures
  • cytidine
  • guanosine
  • hypoxanthine
  • in vitro
  • nucleic acid precursors
  • radioisotopes
  • thymidine
  • uridine

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

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