Uptake of juvenile hormone esterase by pericardial cells of Manduca sexta

Reiji Ichinose, Atsushi Nakamura, Takashi Yamato, Tim F. Booth, Susumu Maeda, Bruce D. Hammock

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

Immunohistochemical studies were conducted to determine tissue(s) which might be involved in the uptake of juvenile hormone esterase (JHE) from larval hemolymph. Purified JHE expressed by a recombinant baculovirus carrying the JHE gene from Heliothis virescens was injected into the hemolymph of second stadium larvae of Manduca sexta. Immunoreactive material detected with specific antibodies against the natural JHE purified by affinity chromatography from the hemolymph of H. virescens was localized only in the dorsal regions of whole larval mounts. Further immunohistochemical studies of whole and dissected larvae at the light and electron microscopic level showed the specific localization of JHE in pericardial cells. Western blot analysis confirmed the localization of injected JHE in pericardial cells and also indicated some apparent degradation of the incorporated JHE. Similar results were obtained with the JHE from H. virescens injected into larvae of H. virescens. These results indicate that pericardial cells are involved in the uptake of injected JHE from insect hemolymph and its degradation.

Original languageEnglish (US)
Pages (from-to)893-904
Number of pages12
JournalInsect Biochemistry and Molecular Biology
Volume22
Issue number8
DOIs
StatePublished - 1992

Keywords

  • Heliothis virescens
  • immunohistochemistry
  • juvenile hormone esterase
  • Manduca sexta
  • pericardial cells

ASJC Scopus subject areas

  • Insect Science
  • Biochemistry

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    Ichinose, R., Nakamura, A., Yamato, T., Booth, T. F., Maeda, S., & Hammock, B. D. (1992). Uptake of juvenile hormone esterase by pericardial cells of Manduca sexta. Insect Biochemistry and Molecular Biology, 22(8), 893-904. https://doi.org/10.1016/0965-1748(92)90116-V