Uptake of GABA by rat brain synaptic vesicles isolated by a new procedure.

Johannes W Hell, P. R. Maycox, H. Stadler, R. Jahn

Research output: Contribution to journalArticle

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Abstract

Uptake of GABA was demonstrated in rat brain synaptic vesicles which were prepared by a new and efficient procedure. The uptake activity co-purified with the synaptic vesicles during the isolation procedure. The purity of the vesicle fraction was rigorously examined by analysis of marker enzymes and marker proteins and also by immunogold electron microscopy using antibodies against p38 (synaptophysin). Contamination by other cellular components was negligible, indicating that GABA uptake by the synaptic vesicle fraction is specific for synaptic vesicles and not due to the presence of other structure possessing GABA uptake or binding activities. GABA uptake was ATP dependent and similar to the uptake of glutamate, which was assayed for a comparison. Both uptake activities were independent of sodium. They were inhibited by the uncoupler carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone, indicating that the energy for the uptake is provided by an electrochemical proton gradient. This gradient is generated by a proton ATPase of the vacuolar type as suggested by the effects of various ATPase inhibitors on neurotransmitter uptake and proton pumping. Competition experiments revealed that the transporters for GABA and glutamate are selective for the respective neurotransmitters.

Original languageEnglish (US)
Pages (from-to)3023-3029
Number of pages7
JournalEMBO Journal
Volume7
Issue number10
StatePublished - Oct 1988
Externally publishedYes

Fingerprint

Synaptic Vesicles
gamma-Aminobutyric Acid
Rats
Brain
Protons
Neurotransmitter Uptake Inhibitors
Neurotransmitter Agents
GABA Plasma Membrane Transport Proteins
Vacuolar Proton-Translocating ATPases
Amino Acid Transport System X-AG
Synaptophysin
Electron microscopy
Adenosine Triphosphatases
Glutamic Acid
Electron Microscopy
Contamination
Adenosine Triphosphate
Sodium
Antibodies
Enzymes

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

Hell, J. W., Maycox, P. R., Stadler, H., & Jahn, R. (1988). Uptake of GABA by rat brain synaptic vesicles isolated by a new procedure. EMBO Journal, 7(10), 3023-3029.

Uptake of GABA by rat brain synaptic vesicles isolated by a new procedure. / Hell, Johannes W; Maycox, P. R.; Stadler, H.; Jahn, R.

In: EMBO Journal, Vol. 7, No. 10, 10.1988, p. 3023-3029.

Research output: Contribution to journalArticle

Hell, JW, Maycox, PR, Stadler, H & Jahn, R 1988, 'Uptake of GABA by rat brain synaptic vesicles isolated by a new procedure.', EMBO Journal, vol. 7, no. 10, pp. 3023-3029.
Hell, Johannes W ; Maycox, P. R. ; Stadler, H. ; Jahn, R. / Uptake of GABA by rat brain synaptic vesicles isolated by a new procedure. In: EMBO Journal. 1988 ; Vol. 7, No. 10. pp. 3023-3029.
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