Upregulation of Runx2 and Osterix during in vitro chondrogenesis of human adipose-derived stromal cells

Jason T. Rich; Ivana Rosová; Jan Nolta; Terence M. Myckatyn; Linda J. Sandell; Audrey McAlinden

doi:10.1016/j.bbrc.2008.05.022

Upregulation of Runx2 and Osterix during in vitro chondrogenesis of human adipose-derived stromal cells

Jason T. Rich, Ivana Rosová, Jan Nolta, Terence M. Myckatyn, Linda J. Sandell, Audrey McAlinden

Hematology and Oncology

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

The aim of this study was to create a gene expression profile to better define the phenotype of human adipose-derived stromal cells (HADSCs) during in vitro chondrogenesis, osteogenesis and adipogenesis. A novel aspect of this work was the analysis of the same subset of genes during HADSC differentiation into all three lineages. Chondrogenic induction resulted in increased mRNA expression of Sox transcription factors, COL2A1, COL10A1, Runx2, and Osterix. This is the first report demonstrating significant upregulation in expression of osteogenesis-related transcription factors Runx2 and Osterix by TGF-β3 induction of HADSCs during in vitro chondrogenesis. These findings suggest that the commonly-used chondrogenic induction reagents promote differentiation suggestive of hypertrophic chondrocytes and osteoblasts. We conclude that alternative strategies are required to induce efficient articular chondrocyte differentiation in order for HADSCs to be of clinical use in cartilage tissue engineering.

Original language	English (US)
Pages (from-to)	230-235
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	372
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2008.05.022
State	Published - Jul 18 2008

Keywords

Chondrogenesis
Human adipose-derived stromal cells (HADSC)
In vitro differentiation
Mesenchymal stem cells
Mesenchymal stromal cell (MSC)
Osterix
Runx2
TGF-β3
Transcription factors

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

Access to Document

10.1016/j.bbrc.2008.05.022

Fingerprint Dive into the research topics of 'Upregulation of Runx2 and Osterix during in vitro chondrogenesis of human adipose-derived stromal cells'. Together they form a unique fingerprint.

Cite this

Upregulation of Runx2 and Osterix during in vitro chondrogenesis of human adipose-derived stromal cells. / Rich, Jason T.; Rosová, Ivana; Nolta, Jan; Myckatyn, Terence M.; Sandell, Linda J.; McAlinden, Audrey.

In: Biochemical and Biophysical Research Communications, Vol. 372, No. 1, 18.07.2008, p. 230-235.

Research output: Contribution to journal › Article › peer-review

@article{91b700708a1f4ef583bd319a9ecac894,

title = "Upregulation of Runx2 and Osterix during in vitro chondrogenesis of human adipose-derived stromal cells",

abstract = "The aim of this study was to create a gene expression profile to better define the phenotype of human adipose-derived stromal cells (HADSCs) during in vitro chondrogenesis, osteogenesis and adipogenesis. A novel aspect of this work was the analysis of the same subset of genes during HADSC differentiation into all three lineages. Chondrogenic induction resulted in increased mRNA expression of Sox transcription factors, COL2A1, COL10A1, Runx2, and Osterix. This is the first report demonstrating significant upregulation in expression of osteogenesis-related transcription factors Runx2 and Osterix by TGF-β3 induction of HADSCs during in vitro chondrogenesis. These findings suggest that the commonly-used chondrogenic induction reagents promote differentiation suggestive of hypertrophic chondrocytes and osteoblasts. We conclude that alternative strategies are required to induce efficient articular chondrocyte differentiation in order for HADSCs to be of clinical use in cartilage tissue engineering.",

keywords = "Chondrogenesis, Human adipose-derived stromal cells (HADSC), In vitro differentiation, Mesenchymal stem cells, Mesenchymal stromal cell (MSC), Osterix, Runx2, TGF-β3, Transcription factors",

author = "Rich, {Jason T.} and Ivana Rosov{\'a} and Jan Nolta and Myckatyn, {Terence M.} and Sandell, {Linda J.} and Audrey McAlinden",

year = "2008",

month = jul,

day = "18",

doi = "10.1016/j.bbrc.2008.05.022",

language = "English (US)",

volume = "372",

pages = "230--235",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Upregulation of Runx2 and Osterix during in vitro chondrogenesis of human adipose-derived stromal cells

AU - Rich, Jason T.

AU - Rosová, Ivana

AU - Nolta, Jan

AU - Myckatyn, Terence M.

AU - Sandell, Linda J.

AU - McAlinden, Audrey

PY - 2008/7/18

Y1 - 2008/7/18

N2 - The aim of this study was to create a gene expression profile to better define the phenotype of human adipose-derived stromal cells (HADSCs) during in vitro chondrogenesis, osteogenesis and adipogenesis. A novel aspect of this work was the analysis of the same subset of genes during HADSC differentiation into all three lineages. Chondrogenic induction resulted in increased mRNA expression of Sox transcription factors, COL2A1, COL10A1, Runx2, and Osterix. This is the first report demonstrating significant upregulation in expression of osteogenesis-related transcription factors Runx2 and Osterix by TGF-β3 induction of HADSCs during in vitro chondrogenesis. These findings suggest that the commonly-used chondrogenic induction reagents promote differentiation suggestive of hypertrophic chondrocytes and osteoblasts. We conclude that alternative strategies are required to induce efficient articular chondrocyte differentiation in order for HADSCs to be of clinical use in cartilage tissue engineering.

AB - The aim of this study was to create a gene expression profile to better define the phenotype of human adipose-derived stromal cells (HADSCs) during in vitro chondrogenesis, osteogenesis and adipogenesis. A novel aspect of this work was the analysis of the same subset of genes during HADSC differentiation into all three lineages. Chondrogenic induction resulted in increased mRNA expression of Sox transcription factors, COL2A1, COL10A1, Runx2, and Osterix. This is the first report demonstrating significant upregulation in expression of osteogenesis-related transcription factors Runx2 and Osterix by TGF-β3 induction of HADSCs during in vitro chondrogenesis. These findings suggest that the commonly-used chondrogenic induction reagents promote differentiation suggestive of hypertrophic chondrocytes and osteoblasts. We conclude that alternative strategies are required to induce efficient articular chondrocyte differentiation in order for HADSCs to be of clinical use in cartilage tissue engineering.

KW - Chondrogenesis

KW - Human adipose-derived stromal cells (HADSC)

KW - In vitro differentiation

KW - Mesenchymal stem cells

KW - Mesenchymal stromal cell (MSC)

KW - Osterix

KW - Runx2

KW - TGF-β3

KW - Transcription factors

UR - http://www.scopus.com/inward/record.url?scp=44649143821&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=44649143821&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2008.05.022

DO - 10.1016/j.bbrc.2008.05.022

M3 - Article

C2 - 18482578

AN - SCOPUS:44649143821

VL - 372

SP - 230

EP - 235

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -

Upregulation of Runx2 and Osterix during in vitro chondrogenesis of human adipose-derived stromal cells

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Cite this