Upregulation of Runx2 and Osterix during in vitro chondrogenesis of human adipose-derived stromal cells

Jason T. Rich; Ivana Rosová; Jan Nolta; Terence M. Myckatyn; Linda J. Sandell; Audrey McAlinden

doi:10.1016/j.bbrc.2008.05.022

Upregulation of Runx2 and Osterix during in vitro chondrogenesis of human adipose-derived stromal cells

Jason T. Rich, Ivana Rosová, Jan Nolta, Terence M. Myckatyn, Linda J. Sandell, Audrey McAlinden

Hematology and Oncology

Research output: Contribution to journal › Article

28 Scopus citations

Abstract

The aim of this study was to create a gene expression profile to better define the phenotype of human adipose-derived stromal cells (HADSCs) during in vitro chondrogenesis, osteogenesis and adipogenesis. A novel aspect of this work was the analysis of the same subset of genes during HADSC differentiation into all three lineages. Chondrogenic induction resulted in increased mRNA expression of Sox transcription factors, COL2A1, COL10A1, Runx2, and Osterix. This is the first report demonstrating significant upregulation in expression of osteogenesis-related transcription factors Runx2 and Osterix by TGF-β3 induction of HADSCs during in vitro chondrogenesis. These findings suggest that the commonly-used chondrogenic induction reagents promote differentiation suggestive of hypertrophic chondrocytes and osteoblasts. We conclude that alternative strategies are required to induce efficient articular chondrocyte differentiation in order for HADSCs to be of clinical use in cartilage tissue engineering.

Original language	English (US)
Pages (from-to)	230-235
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	372
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2008.05.022
State	Published - Jul 18 2008

Keywords

Chondrogenesis
Human adipose-derived stromal cells (HADSC)
In vitro differentiation
Mesenchymal stem cells
Mesenchymal stromal cell (MSC)
Osterix
Runx2
TGF-β3
Transcription factors

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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Rich, J. T., Rosová, I., Nolta, J., Myckatyn, T. M., Sandell, L. J., & McAlinden, A. (2008). Upregulation of Runx2 and Osterix during in vitro chondrogenesis of human adipose-derived stromal cells. Biochemical and Biophysical Research Communications, 372(1), 230-235. https://doi.org/10.1016/j.bbrc.2008.05.022

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abstract = "The aim of this study was to create a gene expression profile to better define the phenotype of human adipose-derived stromal cells (HADSCs) during in vitro chondrogenesis, osteogenesis and adipogenesis. A novel aspect of this work was the analysis of the same subset of genes during HADSC differentiation into all three lineages. Chondrogenic induction resulted in increased mRNA expression of Sox transcription factors, COL2A1, COL10A1, Runx2, and Osterix. This is the first report demonstrating significant upregulation in expression of osteogenesis-related transcription factors Runx2 and Osterix by TGF-β3 induction of HADSCs during in vitro chondrogenesis. These findings suggest that the commonly-used chondrogenic induction reagents promote differentiation suggestive of hypertrophic chondrocytes and osteoblasts. We conclude that alternative strategies are required to induce efficient articular chondrocyte differentiation in order for HADSCs to be of clinical use in cartilage tissue engineering.",

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AU - Nolta, Jan

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AU - Sandell, Linda J.

AU - McAlinden, Audrey

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AB - The aim of this study was to create a gene expression profile to better define the phenotype of human adipose-derived stromal cells (HADSCs) during in vitro chondrogenesis, osteogenesis and adipogenesis. A novel aspect of this work was the analysis of the same subset of genes during HADSC differentiation into all three lineages. Chondrogenic induction resulted in increased mRNA expression of Sox transcription factors, COL2A1, COL10A1, Runx2, and Osterix. This is the first report demonstrating significant upregulation in expression of osteogenesis-related transcription factors Runx2 and Osterix by TGF-β3 induction of HADSCs during in vitro chondrogenesis. These findings suggest that the commonly-used chondrogenic induction reagents promote differentiation suggestive of hypertrophic chondrocytes and osteoblasts. We conclude that alternative strategies are required to induce efficient articular chondrocyte differentiation in order for HADSCs to be of clinical use in cartilage tissue engineering.

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KW - TGF-β3

KW - Transcription factors

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