Abstract
The aim of this study was to create a gene expression profile to better define the phenotype of human adipose-derived stromal cells (HADSCs) during in vitro chondrogenesis, osteogenesis and adipogenesis. A novel aspect of this work was the analysis of the same subset of genes during HADSC differentiation into all three lineages. Chondrogenic induction resulted in increased mRNA expression of Sox transcription factors, COL2A1, COL10A1, Runx2, and Osterix. This is the first report demonstrating significant upregulation in expression of osteogenesis-related transcription factors Runx2 and Osterix by TGF-β3 induction of HADSCs during in vitro chondrogenesis. These findings suggest that the commonly-used chondrogenic induction reagents promote differentiation suggestive of hypertrophic chondrocytes and osteoblasts. We conclude that alternative strategies are required to induce efficient articular chondrocyte differentiation in order for HADSCs to be of clinical use in cartilage tissue engineering.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 230-235 |
| Number of pages | 6 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 372 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jul 18 2008 |
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Keywords
- Chondrogenesis
- Human adipose-derived stromal cells (HADSC)
- In vitro differentiation
- Mesenchymal stem cells
- Mesenchymal stromal cell (MSC)
- Osterix
- Runx2
- TGF-β3
- Transcription factors
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Upregulation of Runx2 and Osterix during in vitro chondrogenesis of human adipose-derived stromal cells. / Rich, Jason T.; Rosová, Ivana; Nolta, Jan; Myckatyn, Terence M.; Sandell, Linda J.; McAlinden, Audrey.
In: Biochemical and Biophysical Research Communications, Vol. 372, No. 1, 18.07.2008, p. 230-235.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Upregulation of Runx2 and Osterix during in vitro chondrogenesis of human adipose-derived stromal cells
AU - Rich, Jason T.
AU - Rosová, Ivana
AU - Nolta, Jan
AU - Myckatyn, Terence M.
AU - Sandell, Linda J.
AU - McAlinden, Audrey
PY - 2008/7/18
Y1 - 2008/7/18
N2 - The aim of this study was to create a gene expression profile to better define the phenotype of human adipose-derived stromal cells (HADSCs) during in vitro chondrogenesis, osteogenesis and adipogenesis. A novel aspect of this work was the analysis of the same subset of genes during HADSC differentiation into all three lineages. Chondrogenic induction resulted in increased mRNA expression of Sox transcription factors, COL2A1, COL10A1, Runx2, and Osterix. This is the first report demonstrating significant upregulation in expression of osteogenesis-related transcription factors Runx2 and Osterix by TGF-β3 induction of HADSCs during in vitro chondrogenesis. These findings suggest that the commonly-used chondrogenic induction reagents promote differentiation suggestive of hypertrophic chondrocytes and osteoblasts. We conclude that alternative strategies are required to induce efficient articular chondrocyte differentiation in order for HADSCs to be of clinical use in cartilage tissue engineering.
AB - The aim of this study was to create a gene expression profile to better define the phenotype of human adipose-derived stromal cells (HADSCs) during in vitro chondrogenesis, osteogenesis and adipogenesis. A novel aspect of this work was the analysis of the same subset of genes during HADSC differentiation into all three lineages. Chondrogenic induction resulted in increased mRNA expression of Sox transcription factors, COL2A1, COL10A1, Runx2, and Osterix. This is the first report demonstrating significant upregulation in expression of osteogenesis-related transcription factors Runx2 and Osterix by TGF-β3 induction of HADSCs during in vitro chondrogenesis. These findings suggest that the commonly-used chondrogenic induction reagents promote differentiation suggestive of hypertrophic chondrocytes and osteoblasts. We conclude that alternative strategies are required to induce efficient articular chondrocyte differentiation in order for HADSCs to be of clinical use in cartilage tissue engineering.
KW - Chondrogenesis
KW - Human adipose-derived stromal cells (HADSC)
KW - In vitro differentiation
KW - Mesenchymal stem cells
KW - Mesenchymal stromal cell (MSC)
KW - Osterix
KW - Runx2
KW - TGF-β3
KW - Transcription factors
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UR - http://www.scopus.com/inward/citedby.url?scp=44649143821&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2008.05.022
DO - 10.1016/j.bbrc.2008.05.022
M3 - Article
C2 - 18482578
AN - SCOPUS:44649143821
VL - 372
SP - 230
EP - 235
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -