Unveiling the metabolic fate of monosaccharides in cell membranes with glycomic and glycoproteomic analyses

Gege Xu, Maurice Wong, Qiongyu Li, Dayoung Park, Zhi Cheng, Carlito B Lebrilla

Research output: Contribution to journalArticle

Abstract

Cell membrane protein glycosylation is dependent on the metabolic state of the cell as well as exogenous nutrients available. Although the metabolism and interconversion of monosaccharides have been well-studied, their incorporation into cell surface glycans and their corresponding glycoproteins remains relatively unknown. In this study, we developed a method to investigate quantitatively the incorporation pathways of dietary saccharides into specific glycans and glycoproteins on the cell membrane by treating intestinal Caco-2 and hepatic KKU-M213 cells with 13C-labeled monosaccharides and characterizing the resulting cell surface glycans and glycopeptides by LC-MS/MS. Time-course studies using uniformly labeled glucose revealed that the rate of incorporation was both glycan-specific and protein-dependent. Comparative studies using different dietary saccharides and multiple cell lines revealed the variance of monosaccharide utilization and interconversion in different tissues and organisms. The robust isotope-labeling and glycan profiling methods can provide a useful tool for differentiating glycosylation pathways and enhance the understanding of how dietary sugar intake affects health.

Original languageEnglish (US)
Pages (from-to)6992-7002
Number of pages11
JournalChemical Science
Volume10
Issue number29
DOIs
StatePublished - Jan 1 2019

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Monosaccharides
Cell membranes
Polysaccharides
Glycosylation
Glycoproteins
Dietary Sucrose
Time and motion study
Glycopeptides
Metabolism
Isotopes
Labeling
Nutrients
Membrane Proteins
Cells
Glycomics
Health
Tissue
Glucose
Proteins

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Unveiling the metabolic fate of monosaccharides in cell membranes with glycomic and glycoproteomic analyses. / Xu, Gege; Wong, Maurice; Li, Qiongyu; Park, Dayoung; Cheng, Zhi; Lebrilla, Carlito B.

In: Chemical Science, Vol. 10, No. 29, 01.01.2019, p. 6992-7002.

Research output: Contribution to journalArticle

Xu, Gege ; Wong, Maurice ; Li, Qiongyu ; Park, Dayoung ; Cheng, Zhi ; Lebrilla, Carlito B. / Unveiling the metabolic fate of monosaccharides in cell membranes with glycomic and glycoproteomic analyses. In: Chemical Science. 2019 ; Vol. 10, No. 29. pp. 6992-7002.
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