Unique antigen recognition by a herpesvirus-specific TCR-γδ cell

Roger Sciammas, Raymond M. Johnson, Anne I. Sperling, William Brady, Peter S. Linsley, Patricia G. Spear, Frank W. Fitch, Jeffrey A. Bluestone

Research output: Contribution to journalArticle

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Abstract

TCR-γδ cells, a T cell subset present in the epithelial and lymphoid tissues, have been implicated in viral and bacterial infections. We have identified a TCR-γδ clone (TgI4.4) that, unlike TCR-αβ cells, recognizes a herpes simplex virus type 1 transmembrane glycoprotein, gl, in an MHC class I- and class II-independent fashion. The TCR of TgI4.4 is composed of rearranged Vδ8 (a Vα2 family member) and Vγ1.2 variable genes, a heterodimeric pair not previously described. Furthermore, anti-Vα2 mAbs are sufficient to block recognition of the gl ligand. Strikingly, anti-gI Abs also are capable of blocking recognition, a phenomena that is very rare in TCR-αβ Ag recognition. Therefore, to dissect the mechanism involved in this unique form of Ag recognition, we constructed a mutant of gl, glt, that lacks cell surface expression upon transfection into APCs. This form of gl was not sufficient for Ag presentation. In contrast, wild-type gl expressed in the Ag-processing mutant cell, RMA-S, is recognized by TgI4.4, suggesting that gl presentation occurs independently of classical Ag-processing pathways. In fact, through the use of a soluble recombinant gI molecule, gI-Ig, we show that TgI4.4 can recognize whole, unprocessed gI protein in the absence of any APCs. These results suggest that there exist alternate and novel forms of TCR Ag recognition, and that the TCR-γδ clone, TgI4.4, may represent a novel T cell subset that, during pathogenic challenge, may respond directly to Ags on the surfaces of bacteria and viruses.

Original languageEnglish (US)
Pages (from-to)5392-5397
Number of pages6
JournalJournal of Immunology
Volume152
Issue number11
StatePublished - Jun 1 1994
Externally publishedYes

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Herpesviridae
Antigens
T-Lymphocyte Subsets
Clone Cells
Human Herpesvirus 1
Lymphoid Tissue
Virus Diseases
Bacterial Infections
Transfection
Glycoproteins
Epithelium
Ligands
Viruses
Bacteria
Genes
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Sciammas, R., Johnson, R. M., Sperling, A. I., Brady, W., Linsley, P. S., Spear, P. G., ... Bluestone, J. A. (1994). Unique antigen recognition by a herpesvirus-specific TCR-γδ cell. Journal of Immunology, 152(11), 5392-5397.

Unique antigen recognition by a herpesvirus-specific TCR-γδ cell. / Sciammas, Roger; Johnson, Raymond M.; Sperling, Anne I.; Brady, William; Linsley, Peter S.; Spear, Patricia G.; Fitch, Frank W.; Bluestone, Jeffrey A.

In: Journal of Immunology, Vol. 152, No. 11, 01.06.1994, p. 5392-5397.

Research output: Contribution to journalArticle

Sciammas, R, Johnson, RM, Sperling, AI, Brady, W, Linsley, PS, Spear, PG, Fitch, FW & Bluestone, JA 1994, 'Unique antigen recognition by a herpesvirus-specific TCR-γδ cell', Journal of Immunology, vol. 152, no. 11, pp. 5392-5397.
Sciammas R, Johnson RM, Sperling AI, Brady W, Linsley PS, Spear PG et al. Unique antigen recognition by a herpesvirus-specific TCR-γδ cell. Journal of Immunology. 1994 Jun 1;152(11):5392-5397.
Sciammas, Roger ; Johnson, Raymond M. ; Sperling, Anne I. ; Brady, William ; Linsley, Peter S. ; Spear, Patricia G. ; Fitch, Frank W. ; Bluestone, Jeffrey A. / Unique antigen recognition by a herpesvirus-specific TCR-γδ cell. In: Journal of Immunology. 1994 ; Vol. 152, No. 11. pp. 5392-5397.
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