Understanding Immune Resistance to Infectious Bronchitis Using Major Histocompatibility Complex Chicken Lines

A. P. Da Silva, R. Hauck, H. Zhou, Rodrigo A Gallardo

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Genetic resistance or susceptibility to infectious diseases has been largely associated with the avian major histocompatibility complex (MHC) genes. Our goal was to determine resistance and susceptibility of MHC B haplotype in congenic and inbred chicken lines in order to establish a resistant-susceptible model. Eight congenic lines (253/B18, 254/B15, 330/B21, 312/B24, 331/B2, 335/B19, 336/B21, and 342/BO), two inbred lines (003/B17 and 077/B19), and three commercial lines (white leghorn, brown layers, and broilers) were used in two experiments. We analyzed and compared immunologic responses and the effect of challenge by measuring viral load, IgG and IgA humoral responses, histopathology and histomorphometry, clinical signs, and immune cell populations in the different MHC B haplotype lines. We found that respiratory signs, tracheal deciliation and inflammation, airsacculitis, viral shedding in tears, and local humoral responses were good parameters to determine resistance or susceptibility. Based on these results, we identified 331/B2 as the most resistant and 335/B19 as the most susceptible congenic chicken lines. These two lines will be used as an animal model in subsequent experiments to understand the mechanisms by which the immune system in chickens generates resistance to infectious bronchitis virus.

Original languageEnglish (US)
Pages (from-to)358-365
Number of pages8
JournalAvian Diseases
Volume61
Issue number3
DOIs
StatePublished - Sep 1 2017

Fingerprint

infectious bronchitis
Bronchitis
major histocompatibility complex
Major Histocompatibility Complex
Chickens
chickens
humoral immunity
Haplotypes
haplotypes
Infectious bronchitis virus
Virus Shedding
viral shedding
genetic resistance
White Leghorn
viral load
Viral Load
Tears
histopathology
Immunoglobulin A
inbred lines

Keywords

  • Infectious bronchitis virus
  • Major histocompatibility complex
  • Resistance
  • Susceptibility

ASJC Scopus subject areas

  • Food Animals
  • Animal Science and Zoology
  • Immunology and Microbiology(all)

Cite this

Understanding Immune Resistance to Infectious Bronchitis Using Major Histocompatibility Complex Chicken Lines. / Da Silva, A. P.; Hauck, R.; Zhou, H.; Gallardo, Rodrigo A.

In: Avian Diseases, Vol. 61, No. 3, 01.09.2017, p. 358-365.

Research output: Contribution to journalArticle

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