Ultrastructure of the nonciliated bronchiolar epithelial (clara) cell of mammalian lung

II. A comparison of horse, steer, sheep, dog, and cat

Charles Plopper, Andrew T. Mariassy, Lila H. Hill

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Two morphologic characteristics have been used to define the nonciliated bronchiolar epithelial cell: abundant agranular endoplasmic reticulum (AER) and membrane-bound ovoid granules. To assess the ultrastructural homogeneity of this cell type in the lungs of large domestic mammals used as experimental models in pulmonary research, we evaluated lungs of horse, steer, sheep, dog, and cat. Bronchioles of known anatomic location were examined by electron microscopy following fixation by airway infusion at standard pressure and processing by selective embedding techniques. Nonciliated bronchiolar epithelium of the horse and sheep had numerous ovoid granules (averaging above 15 per cell) and abundant AER. Granules were scarce (averaging less than 2 per cell) in steer and dog and absent in cat. AER was minimal in these species compared to horse and sheep. Glycogen was the dominant cytoplasmic feature in steer, dog, and cat, variable in sheep and rare in horse. Large mitochondria with few cristae and densely staining matrix were present only in cat. We concluded that nonciliated bronchiolar cells of horse and sheep were similar in essential features to this cell type in laboratory mammals, having granules and AER in abundance, while those of steer, dog, and cat were not.

Original languageEnglish (US)
Pages (from-to)155-169
Number of pages15
JournalExperimental Lung Research
Volume1
Issue number2
DOIs
StatePublished - Jan 1 1980
Externally publishedYes

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Mammals
Smooth Endoplasmic Reticulum
Horses
Sheep
Cats
Epithelial Cells
Dogs
Lung
Mitochondria
Glycogen
Electron microscopy
Membranes
Bronchioles
Processing
Electron Microscopy
Theoretical Models
Epithelium
Staining and Labeling
Pressure
Research

ASJC Scopus subject areas

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry

Cite this

Ultrastructure of the nonciliated bronchiolar epithelial (clara) cell of mammalian lung : II. A comparison of horse, steer, sheep, dog, and cat. / Plopper, Charles; Mariassy, Andrew T.; Hill, Lila H.

In: Experimental Lung Research, Vol. 1, No. 2, 01.01.1980, p. 155-169.

Research output: Contribution to journalArticle

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