Ultrastructure of the intra-erythrocytic stage of Theileria species from cattle and waterbuck

Donald W. Fawcett, Patricia A Conrad, Jan G. Grootenhuis, Subhash P. Morzaria

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A transmission electron microscopic study of the intra-erythrocytic stages of a pathogenic Theileria parva from cattle and a previously uncharacterized Theileria sp. from waterbuck (Kobus defassa) in Kenya revealed several novel ultrastructural features, associated with feeding and multiplication, in these parasites. In trophozoites a connecting channel was observed between the parasite's cytostome and its intracytoplasmic food vacuole. In some cases the limiting membrane of the food vacuole was seen to be continuous with a close-meshed network of membrane-bounded, anastomosing tubules. This labyrinthine structure, which has not been described previously, may function as a digestive organelle in theilerial trophozoites. Electron micrographs also revealed the mode of intra-erythrocytic multiplication of these parasites in vivo. Prior to division, electron-dense cisternae and rhoptries appeared beneath the parasite's plasmalemmal membrane, marking the sites of merozoite formation. From a single parasite, a maximum of four merozoites were formed by schizogonous division and subsequently separated from a residual body by constriction at the base of each merozoite. In addition, observations on two double-membraned organelles seen in trophozoites and the intra-erythrocytic crystalline structures associated with Theileria sp. in waterbuck are reported.

Original languageEnglish (US)
Pages (from-to)643-655
Number of pages13
JournalTissue and Cell
Volume19
Issue number5
DOIs
StatePublished - 1987
Externally publishedYes

Fingerprint

erythrocytic stage
Theileria
ultrastructure
Parasites
Merozoites
Trophozoites
trophozoites
merozoites
parasites
cattle
electrons
Electrons
Vacuoles
Organelles
vacuoles
Membranes
organelles
Theileria parva
Kobus
Food

Keywords

  • merozoites
  • piroplasms
  • protozoa
  • schizogony
  • Theileria parva
  • waterbuck

ASJC Scopus subject areas

  • Insect Science
  • Cell Biology

Cite this

Ultrastructure of the intra-erythrocytic stage of Theileria species from cattle and waterbuck. / Fawcett, Donald W.; Conrad, Patricia A; Grootenhuis, Jan G.; Morzaria, Subhash P.

In: Tissue and Cell, Vol. 19, No. 5, 1987, p. 643-655.

Research output: Contribution to journalArticle

Fawcett, Donald W. ; Conrad, Patricia A ; Grootenhuis, Jan G. ; Morzaria, Subhash P. / Ultrastructure of the intra-erythrocytic stage of Theileria species from cattle and waterbuck. In: Tissue and Cell. 1987 ; Vol. 19, No. 5. pp. 643-655.
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