Ultrasound-enhanced thrombolysis at 20 kHz with air-filled and perfluorocarbon-filled contrast bispheres

Milind R. Dhond, Thanh T. Nguyen, Chris Dolan, Greg Pulido, William J Bommer

Research output: Contribution to journalArticlepeer-review

23 Scopus citations


Background: Ultrasound (US) at low frequencies has been shown to enhance clot lysis by itself and in the presence of urokinase (UK). The comparative effects of air-filled versus perfluorocarbon-filled polymer bispheres in enhancing this effect have not been previously demonstrated. Methods: Freshly drawn human blood was incubated at 37°C for 2 hours, and the subsequent formed clot was dried and weighed. It was then exposed to saline control, saline + UK (10,000 IU), saline + UK + US, saline + UK + US + low shell-strength polymer bispheres (PB1), saline + UK + US + high shell-strength polymer bispheres (PB2), and perfluorocarbon-filled high shell-strength polymer bipsheres (PB3) for a total of 6 minutes. Clots were removed and weighed to determine the percentage of thrombolysis. Results: The percentage of clot lysis for each study group was as follows: saline 18.5% ± 4%, US alone 22.2% ± 5%, UK alone 21.9% ± 4%, US+UK 32.2% ± 8% (P < .05 compared with UK alone), US+UK+PB1 36.9% ± 8%, US+UK+PB2 34.3% ± 8%, and US+UK+PB3 45.0% ± 11% (P < .05 compared with US+UK, P < .05 compared with US+UK+PB2). Conclusion: Ultrasound at 20 kHz significantly enhances clot lysis. The addition of perfluorocarbon-filled bispheres increased this effect more significantly than did the addition of air-filled polymer bispheres.

Original languageEnglish (US)
Pages (from-to)1025-1029
Number of pages5
JournalJournal of the American Society of Echocardiography
Issue number11
StatePublished - 2000

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Cardiology and Cardiovascular Medicine


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