Ultrasound detection and characterization of polycystic kidney disease in a mouse model

Rachel E Pollard, Reem Yunis, Dietmar Kültz, Phillip Martin, Stephen M Griffey, Katherine Ferrara

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We sought to use ultrasonography to quantify renal size and echogenicity in a mouse model of polycystic kidney disease. We imaged 36 wild-type (WT) and juvenile cystic kidney (jck) mice by using a standard ultrasound unit and 10-5 MHz linear transducer. Mice were imaged at 3 (6 WT, 7 jck), 6 (7 WT, 5 jck), and 9 (6 WT, 5 jck) wk of age. Kidney length, width, and height were recorded for volume calculation. Sagittal images of both kidneys were recorded for assessment of intensity. Quantitative values were obtained from areas of similar depth and gain settings. Kidney and liver intensities were determined for calculation of their ratio. Representative histologic kidney sections were stained with hematoxylin and eosin and digitized for calculation of cyst number, mean cyst area, and percentage cystic area. We found that renal volume was greater in jck than WT mice at 3 (P < 0.0001), 6 (P < 0.0001), and 9 (P < 0.0001) wk of age. In addition, kidney intensity and kidney:liver ratio were higher in jck than WT mice at 3 (P < 0.002 for both parameters), 6 (P < 0.04), and 9 wk (P < 0.008). Kidneys with smaller mean cyst size and less percentage cystic space had higher intensity values. We therefore conclude that ultrasound measures of renal volume and intensity can noninvasively identify jck-affected mice as early as 3 wk of age. Cortical intensity is greater in jck versus WT mice and appears affected by percentage cyst area and mean cyst size.

Original languageEnglish (US)
Pages (from-to)215-221
Number of pages7
JournalComparative Medicine
Volume56
Issue number3
StatePublished - Jun 2006

Fingerprint

Polycystic Kidney Diseases
disease models
Cystic Kidney Diseases
animal models
Ultrasonics
kidneys
Kidney
Liver
Ultrasonography
Cysts
Hematoxylin
Eosine Yellowish-(YS)
Transducers
mice
polycystic kidney diseases
liver

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Ultrasound detection and characterization of polycystic kidney disease in a mouse model. / Pollard, Rachel E; Yunis, Reem; Kültz, Dietmar; Martin, Phillip; Griffey, Stephen M; Ferrara, Katherine.

In: Comparative Medicine, Vol. 56, No. 3, 06.2006, p. 215-221.

Research output: Contribution to journalArticle

Pollard, Rachel E ; Yunis, Reem ; Kültz, Dietmar ; Martin, Phillip ; Griffey, Stephen M ; Ferrara, Katherine. / Ultrasound detection and characterization of polycystic kidney disease in a mouse model. In: Comparative Medicine. 2006 ; Vol. 56, No. 3. pp. 215-221.
@article{00e8f89be025426689cc257bbf4f20c6,
title = "Ultrasound detection and characterization of polycystic kidney disease in a mouse model",
abstract = "We sought to use ultrasonography to quantify renal size and echogenicity in a mouse model of polycystic kidney disease. We imaged 36 wild-type (WT) and juvenile cystic kidney (jck) mice by using a standard ultrasound unit and 10-5 MHz linear transducer. Mice were imaged at 3 (6 WT, 7 jck), 6 (7 WT, 5 jck), and 9 (6 WT, 5 jck) wk of age. Kidney length, width, and height were recorded for volume calculation. Sagittal images of both kidneys were recorded for assessment of intensity. Quantitative values were obtained from areas of similar depth and gain settings. Kidney and liver intensities were determined for calculation of their ratio. Representative histologic kidney sections were stained with hematoxylin and eosin and digitized for calculation of cyst number, mean cyst area, and percentage cystic area. We found that renal volume was greater in jck than WT mice at 3 (P < 0.0001), 6 (P < 0.0001), and 9 (P < 0.0001) wk of age. In addition, kidney intensity and kidney:liver ratio were higher in jck than WT mice at 3 (P < 0.002 for both parameters), 6 (P < 0.04), and 9 wk (P < 0.008). Kidneys with smaller mean cyst size and less percentage cystic space had higher intensity values. We therefore conclude that ultrasound measures of renal volume and intensity can noninvasively identify jck-affected mice as early as 3 wk of age. Cortical intensity is greater in jck versus WT mice and appears affected by percentage cyst area and mean cyst size.",
author = "Pollard, {Rachel E} and Reem Yunis and Dietmar K{\"u}ltz and Phillip Martin and Griffey, {Stephen M} and Katherine Ferrara",
year = "2006",
month = "6",
language = "English (US)",
volume = "56",
pages = "215--221",
journal = "Comparative Medicine",
issn = "1532-0820",
publisher = "American Association for Laboratory Animal Science",
number = "3",

}

TY - JOUR

T1 - Ultrasound detection and characterization of polycystic kidney disease in a mouse model

AU - Pollard, Rachel E

AU - Yunis, Reem

AU - Kültz, Dietmar

AU - Martin, Phillip

AU - Griffey, Stephen M

AU - Ferrara, Katherine

PY - 2006/6

Y1 - 2006/6

N2 - We sought to use ultrasonography to quantify renal size and echogenicity in a mouse model of polycystic kidney disease. We imaged 36 wild-type (WT) and juvenile cystic kidney (jck) mice by using a standard ultrasound unit and 10-5 MHz linear transducer. Mice were imaged at 3 (6 WT, 7 jck), 6 (7 WT, 5 jck), and 9 (6 WT, 5 jck) wk of age. Kidney length, width, and height were recorded for volume calculation. Sagittal images of both kidneys were recorded for assessment of intensity. Quantitative values were obtained from areas of similar depth and gain settings. Kidney and liver intensities were determined for calculation of their ratio. Representative histologic kidney sections were stained with hematoxylin and eosin and digitized for calculation of cyst number, mean cyst area, and percentage cystic area. We found that renal volume was greater in jck than WT mice at 3 (P < 0.0001), 6 (P < 0.0001), and 9 (P < 0.0001) wk of age. In addition, kidney intensity and kidney:liver ratio were higher in jck than WT mice at 3 (P < 0.002 for both parameters), 6 (P < 0.04), and 9 wk (P < 0.008). Kidneys with smaller mean cyst size and less percentage cystic space had higher intensity values. We therefore conclude that ultrasound measures of renal volume and intensity can noninvasively identify jck-affected mice as early as 3 wk of age. Cortical intensity is greater in jck versus WT mice and appears affected by percentage cyst area and mean cyst size.

AB - We sought to use ultrasonography to quantify renal size and echogenicity in a mouse model of polycystic kidney disease. We imaged 36 wild-type (WT) and juvenile cystic kidney (jck) mice by using a standard ultrasound unit and 10-5 MHz linear transducer. Mice were imaged at 3 (6 WT, 7 jck), 6 (7 WT, 5 jck), and 9 (6 WT, 5 jck) wk of age. Kidney length, width, and height were recorded for volume calculation. Sagittal images of both kidneys were recorded for assessment of intensity. Quantitative values were obtained from areas of similar depth and gain settings. Kidney and liver intensities were determined for calculation of their ratio. Representative histologic kidney sections were stained with hematoxylin and eosin and digitized for calculation of cyst number, mean cyst area, and percentage cystic area. We found that renal volume was greater in jck than WT mice at 3 (P < 0.0001), 6 (P < 0.0001), and 9 (P < 0.0001) wk of age. In addition, kidney intensity and kidney:liver ratio were higher in jck than WT mice at 3 (P < 0.002 for both parameters), 6 (P < 0.04), and 9 wk (P < 0.008). Kidneys with smaller mean cyst size and less percentage cystic space had higher intensity values. We therefore conclude that ultrasound measures of renal volume and intensity can noninvasively identify jck-affected mice as early as 3 wk of age. Cortical intensity is greater in jck versus WT mice and appears affected by percentage cyst area and mean cyst size.

UR - http://www.scopus.com/inward/record.url?scp=33745047028&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33745047028&partnerID=8YFLogxK

M3 - Article

C2 - 16774131

AN - SCOPUS:33745047028

VL - 56

SP - 215

EP - 221

JO - Comparative Medicine

JF - Comparative Medicine

SN - 1532-0820

IS - 3

ER -