Abstract
Matrigel, a basement membrane extract, has been extensively used in in vivo angiogenesis. Contrast ultrasound imaging (CUI) of implanted Matrigel plugs with (+bFGF) and without basic fibroblast growth factor (-bFGF) was performed 7 and 14 d after implantation, followed by histologic analysis. Statistically significant differences between +bFGF and -bFGF plugs were apparent at d 7 in both plug size and contrast enhancement (both p < 0.05). Histopathology revealed differences in microvessel density (MVD) between +bFGF and -bFGF at d 7 and d 14. A significant correlation between MVD and both power Doppler contrast-enhanced area (r = 0.65, p < 0.05) and fraction of plug enhanced (r = 0.59, p < 0.05) was present. CUI of Matrigel plugs was shown to be a robust method for distinguishing between two different angiogenic states. Ultrasound measurements of blood flow in the plugs correlated with MVD, a histologic technique used to quantify tumor angiogenesis. (E-mail: smstieger@ucdavis.edu).
| Original language | English (US) |
|---|---|
| Pages (from-to) | 673-681 |
| Number of pages | 9 |
| Journal | Ultrasound in Medicine and Biology |
| Volume | 32 |
| Issue number | 5 |
| DOIs | |
| State | Published - May 2006 |
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Keywords
- Angiogenesis
- Contrast ultrasound imaging
- Matrigel
- Power Doppler
- Rat
- Tumor
ASJC Scopus subject areas
- Radiology Nuclear Medicine and imaging
Cite this
Ultrasound assessment of angiogenesis in a matrigel model in rats. / Stieger, Susanne M.; Bloch, Susannah H.; Foreman, Oded; Wisner, Erik R; Ferrara, Katherine W.; Dayton, Paul A.
In: Ultrasound in Medicine and Biology, Vol. 32, No. 5, 05.2006, p. 673-681.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Ultrasound assessment of angiogenesis in a matrigel model in rats
AU - Stieger, Susanne M.
AU - Bloch, Susannah H.
AU - Foreman, Oded
AU - Wisner, Erik R
AU - Ferrara, Katherine W.
AU - Dayton, Paul A.
PY - 2006/5
Y1 - 2006/5
N2 - Matrigel, a basement membrane extract, has been extensively used in in vivo angiogenesis. Contrast ultrasound imaging (CUI) of implanted Matrigel plugs with (+bFGF) and without basic fibroblast growth factor (-bFGF) was performed 7 and 14 d after implantation, followed by histologic analysis. Statistically significant differences between +bFGF and -bFGF plugs were apparent at d 7 in both plug size and contrast enhancement (both p < 0.05). Histopathology revealed differences in microvessel density (MVD) between +bFGF and -bFGF at d 7 and d 14. A significant correlation between MVD and both power Doppler contrast-enhanced area (r = 0.65, p < 0.05) and fraction of plug enhanced (r = 0.59, p < 0.05) was present. CUI of Matrigel plugs was shown to be a robust method for distinguishing between two different angiogenic states. Ultrasound measurements of blood flow in the plugs correlated with MVD, a histologic technique used to quantify tumor angiogenesis. (E-mail: smstieger@ucdavis.edu).
AB - Matrigel, a basement membrane extract, has been extensively used in in vivo angiogenesis. Contrast ultrasound imaging (CUI) of implanted Matrigel plugs with (+bFGF) and without basic fibroblast growth factor (-bFGF) was performed 7 and 14 d after implantation, followed by histologic analysis. Statistically significant differences between +bFGF and -bFGF plugs were apparent at d 7 in both plug size and contrast enhancement (both p < 0.05). Histopathology revealed differences in microvessel density (MVD) between +bFGF and -bFGF at d 7 and d 14. A significant correlation between MVD and both power Doppler contrast-enhanced area (r = 0.65, p < 0.05) and fraction of plug enhanced (r = 0.59, p < 0.05) was present. CUI of Matrigel plugs was shown to be a robust method for distinguishing between two different angiogenic states. Ultrasound measurements of blood flow in the plugs correlated with MVD, a histologic technique used to quantify tumor angiogenesis. (E-mail: smstieger@ucdavis.edu).
KW - Angiogenesis
KW - Contrast ultrasound imaging
KW - Matrigel
KW - Power Doppler
KW - Rat
KW - Tumor
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UR - http://www.scopus.com/inward/citedby.url?scp=33646133867&partnerID=8YFLogxK
U2 - 10.1016/j.ultrasmedbio.2005.12.008
DO - 10.1016/j.ultrasmedbio.2005.12.008
M3 - Article
C2 - 16677927
AN - SCOPUS:33646133867
VL - 32
SP - 673
EP - 681
JO - Ultrasound in Medicine and Biology
JF - Ultrasound in Medicine and Biology
SN - 0301-5629
IS - 5
ER -