Ultrasound assessment of angiogenesis in a matrigel model in rats

Susanne M. Stieger, Susannah H. Bloch, Oded Foreman, Erik R Wisner, Katherine W. Ferrara, Paul A. Dayton

Research output: Contribution to journalArticlepeer-review

35 Scopus citations


Matrigel, a basement membrane extract, has been extensively used in in vivo angiogenesis. Contrast ultrasound imaging (CUI) of implanted Matrigel plugs with (+bFGF) and without basic fibroblast growth factor (-bFGF) was performed 7 and 14 d after implantation, followed by histologic analysis. Statistically significant differences between +bFGF and -bFGF plugs were apparent at d 7 in both plug size and contrast enhancement (both p < 0.05). Histopathology revealed differences in microvessel density (MVD) between +bFGF and -bFGF at d 7 and d 14. A significant correlation between MVD and both power Doppler contrast-enhanced area (r = 0.65, p < 0.05) and fraction of plug enhanced (r = 0.59, p < 0.05) was present. CUI of Matrigel plugs was shown to be a robust method for distinguishing between two different angiogenic states. Ultrasound measurements of blood flow in the plugs correlated with MVD, a histologic technique used to quantify tumor angiogenesis. (E-mail: smstieger@ucdavis.edu).

Original languageEnglish (US)
Pages (from-to)673-681
Number of pages9
JournalUltrasound in Medicine and Biology
Issue number5
StatePublished - May 2006


  • Angiogenesis
  • Contrast ultrasound imaging
  • Matrigel
  • Power Doppler
  • Rat
  • Tumor

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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