Ultrasonographic and histologic evaluation of medial and middle patellar ligaments in exercised horses following injection with ethanolamine oleate and 2% iodine in almond oil

Linda M. Van Hoogmoed, Dalen W. Agnew, Mary B Whitcomb, Dallas W. Hyde, Melinda H. MacDonald, Jack R. Snyder

Research output: Contribution to journalArticle

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Abstract

Objective.-To describe the ultrasonographic and quantitative histologic effect of injecting 2% iodine in almond oil (IAO) and ethanolamine oleate (EO) in the medial and middle patellar ligaments of horses and to determine whether a difference in response exists between IAO and EO treatment. Animals.-10 healthy horses. Procedure.-In 5 horses, the medial and middle patellar ligaments of 1 limb were injected with EO, whereas IAO was injected in the medial and middle patellar ligaments of another 5 horses. Ultrasonographic evaluation was performed on the experimental and control limb before injection of IAO and EO and prior to euthanasia to determine cross-sectional area and evaluate fiber pattern. The patellar ligaments were harvested 2 weeks after injection and examined histologically to evaluate the inflammatory response, fibroplasia, and chondroid metaplasia. Results.-Injection of the patellar ligaments with IAO resulted in a greater increase in cross-sectional area on ultrasonography than EO. Both agents caused a decrease in echogenicity of the ligament. Histologically, significantly greater infiltration of inflammatory cells and fibroplasia developed after injection with IAO, compared with EO. Both agents resulted in significantly greater fibroplasia relative to control specimens. Conclusions and Clinical Relevance.-Injection of the medial and middle patellar ligaments with IAO induces more severe inflammation and fibroplasia than EO. Maturation of the inflammatory and fibrous response may contribute to resolution or attenuation of upward fixation of the patella by subsequent stiffening of the ligaments.

Original languageEnglish (US)
Pages (from-to)738-743
Number of pages6
JournalAmerican Journal of Veterinary Research
Volume63
Issue number5
StatePublished - 2002

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almond oil
Patellar Ligament
ethanolamine
ligaments
iodine
Iodine
Horses
oleic acid
injection
horses
Injections
Ligaments
limbs (animal)
Extremities
inflammation
Euthanasia
Patella
metaplasia
Metaplasia
ethanolamine oleate

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Ultrasonographic and histologic evaluation of medial and middle patellar ligaments in exercised horses following injection with ethanolamine oleate and 2% iodine in almond oil. / Van Hoogmoed, Linda M.; Agnew, Dalen W.; Whitcomb, Mary B; Hyde, Dallas W.; MacDonald, Melinda H.; Snyder, Jack R.

In: American Journal of Veterinary Research, Vol. 63, No. 5, 2002, p. 738-743.

Research output: Contribution to journalArticle

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abstract = "Objective.-To describe the ultrasonographic and quantitative histologic effect of injecting 2{\%} iodine in almond oil (IAO) and ethanolamine oleate (EO) in the medial and middle patellar ligaments of horses and to determine whether a difference in response exists between IAO and EO treatment. Animals.-10 healthy horses. Procedure.-In 5 horses, the medial and middle patellar ligaments of 1 limb were injected with EO, whereas IAO was injected in the medial and middle patellar ligaments of another 5 horses. Ultrasonographic evaluation was performed on the experimental and control limb before injection of IAO and EO and prior to euthanasia to determine cross-sectional area and evaluate fiber pattern. The patellar ligaments were harvested 2 weeks after injection and examined histologically to evaluate the inflammatory response, fibroplasia, and chondroid metaplasia. Results.-Injection of the patellar ligaments with IAO resulted in a greater increase in cross-sectional area on ultrasonography than EO. Both agents caused a decrease in echogenicity of the ligament. Histologically, significantly greater infiltration of inflammatory cells and fibroplasia developed after injection with IAO, compared with EO. Both agents resulted in significantly greater fibroplasia relative to control specimens. Conclusions and Clinical Relevance.-Injection of the medial and middle patellar ligaments with IAO induces more severe inflammation and fibroplasia than EO. Maturation of the inflammatory and fibrous response may contribute to resolution or attenuation of upward fixation of the patella by subsequent stiffening of the ligaments.",
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AU - Hyde, Dallas W.

AU - MacDonald, Melinda H.

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