Ultrasonic mechanical relaxation imaging of pH in biopolymers

Mallika Sridhar, Huini Du, Claire Pellot-Barakat, Scott I. Simon, Michael F. Insana

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Scopus citations

Abstract

Ultrasonic Mechanical Relaxation (UMR) imaging is a new research technique for visualizing viscoelastic properties of tumors. Tissues behave mechanically as water-based polymers, similar to gelatin, with time-varying viscoelastic properties that depend on the chemical environment. We hypothesized that changes in pH, alter the polymer-fiber surface charge density that determines extent of polymer cross-linking. Gelatin samples with similar material properties and variable pH were prepared. A cone-plate viscometer measured the elastic as well as the viscous response of the polymer to a shear stress stimulus in the pH range of 6 to 8. To image local pH changes, two homogeneous gelatin samples were constructed, one made from buffered saline and the other was unbuffered. 0.05ml NaOH (pH 12) was injected into both samples and subsequent dynamic changes were imaged using UMR methods at 5, 20 and 50 minutes. UMR images include elastic strain and viscous creep relaxation maps produced by applying a compressive step-stress stimulus while recording RF echo frames at a high rate. Estimated local displacements occurring between frames in the echo sequence yield strain images. Relaxation parameters are estimated and mapped for each pixel using the strain time series to produce parametric UMR images. Viscometer experiments indicate that the viscoelastic properties of gelatin vary with pH. Also, elastic strain and viscous creep UMR images show contrast in the region of pH change. These results suggest that UMR methods can be used to explore the microenvironments of living tumors, where their viscoelastic properties are influenced by changes in pO 2, pH and collagen density that predict metastatic potential and resistance to treatments.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
EditorsW.F. Walker, S.Y. Emelianov
Pages202-211
Number of pages10
Volume5
Edition27
DOIs
StatePublished - 2004
EventMedical Imaging 2004 - Ultrasonic Imaging and Signal Processing - San Diego, CA, United States
Duration: Feb 18 2004Feb 19 2004

Other

OtherMedical Imaging 2004 - Ultrasonic Imaging and Signal Processing
CountryUnited States
CitySan Diego, CA
Period2/18/042/19/04

Keywords

  • Biopolymers
  • pH Imaging
  • UMR Imaging
  • Viscoelasticity

ASJC Scopus subject areas

  • Engineering(all)

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  • Cite this

    Sridhar, M., Du, H., Pellot-Barakat, C., Simon, S. I., & Insana, M. F. (2004). Ultrasonic mechanical relaxation imaging of pH in biopolymers. In W. F. Walker, & S. Y. Emelianov (Eds.), Progress in Biomedical Optics and Imaging - Proceedings of SPIE (27 ed., Vol. 5, pp. 202-211). [24] https://doi.org/10.1117/12.536975