Ultrahigh speed spectral / Fourier domain OCT ophthalmic imaging at 70,000 to 312,500 axial scans per second

Benjamin Potsaid, Iwona Gorczynska, Vivek Srinivasan, Yueli Chen, James Jiang, Alex Cable, James G. Fujimoto

Research output: Contribution to journalArticle

321 Citations (Scopus)

Abstract

We demonstrate ultrahigh speed spectral / Fourier domain optical coherence tomography (OCT) using an ultrahigh speed CMOS line scan camera at rates of 70,000 - 312,500 axial scans per second. Several design configurations are characterized to illustrate trade-offs between acquisition speed, resolution, imaging range, sensitivity and sensitivity roll-off performance. Ultrahigh resolution OCT with 2.5 - 3.0 micron axial image resolution is demonstrated at -100,000 axial scans per second. A high resolution spectrometer design improves sensitivity roll-off and imaging range performance, trading off imaging speed to 70,000 axial scans per second. Ultrahigh speed imaging at > 300,000 axial scans per second with standard image resolution is also demonstrated. Ophthalmic OCT imaging of the normal human retina is investigated. The high acquisition speeds enable dense raster scanning to acquire densely sampled volumetric three dimensional OCT (3D-OCT) data sets of the macula and optic disc with minimal motion artifacts. Imaging with -8 - 9 micron axial resolution at 250,000 axial scans per second, a 512 × 512 × 400 voxel volumetric 3D-OCT data set can be acquired in only -1.3 seconds. Orthogonal registration scans are used to register OCT raster scans and remove residual axial eye motion, resulting in 3D-OCT data sets which preserve retinal topography. Rapid repetitive imaging over small volumes can visualize small retinal features without motion induced distortions and enables volume registration to remove eye motion. Cone photoreceptors in some regions of the retina can be visualized without adaptive optics or active eye tracking. Rapid repetitive imaging of 3D volumes also provides dynamic volumetric information (4D-OCT) which is shown to enhance visualization of retinal capillaries and should enable functional imaging. Improvements in the speed and performance of 3D-OCT volumetric imaging promise to enable earlier diagnosis and improved monitoring of disease progression and response to therapy in ophthalmology, as well as have a wide range of research and clinical applications in other areas.

Original languageEnglish (US)
Pages (from-to)15149-15169
Number of pages21
JournalOptics Express
Volume16
Issue number19
DOIs
StatePublished - Sep 15 2008
Externally publishedYes

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tomography
retina
image resolution
sensitivity
acquisition
raster scanning
ophthalmology
photoreceptors
registers
adaptive optics
progressions
artifacts
therapy
CMOS
cones
topography
cameras
optics
spectrometers
high resolution

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Ultrahigh speed spectral / Fourier domain OCT ophthalmic imaging at 70,000 to 312,500 axial scans per second. / Potsaid, Benjamin; Gorczynska, Iwona; Srinivasan, Vivek; Chen, Yueli; Jiang, James; Cable, Alex; Fujimoto, James G.

In: Optics Express, Vol. 16, No. 19, 15.09.2008, p. 15149-15169.

Research output: Contribution to journalArticle

Potsaid, B, Gorczynska, I, Srinivasan, V, Chen, Y, Jiang, J, Cable, A & Fujimoto, JG 2008, 'Ultrahigh speed spectral / Fourier domain OCT ophthalmic imaging at 70,000 to 312,500 axial scans per second', Optics Express, vol. 16, no. 19, pp. 15149-15169. https://doi.org/10.1364/OE.16.015149
Potsaid, Benjamin ; Gorczynska, Iwona ; Srinivasan, Vivek ; Chen, Yueli ; Jiang, James ; Cable, Alex ; Fujimoto, James G. / Ultrahigh speed spectral / Fourier domain OCT ophthalmic imaging at 70,000 to 312,500 axial scans per second. In: Optics Express. 2008 ; Vol. 16, No. 19. pp. 15149-15169.
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