Ultrahigh speed imaging of the rat retina using ultrahigh resolution spectral / Fourier domain OCT

Jonathan J. Liu, Benjamin Potsaid, Yueli Chen, Iwona Gorczynska, Vivek Srinivasan, Jay S. Duker, James G. Fujimoto

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We performed OCT imaging of the rat retina at 70,000 axial scans per second with ∼3 μm axial resolution. Three-dimensional OCT (3D-OCT) data sets of the rat retina were acquired. The high speed and high density data sets enable improved en face visualization by reducing eye motion artifacts and improve Doppler OCT measurements. Minimal motion artifacts were visible and the OCT fundus images offer more precise registration of individual OCT images to retinal fundus features. Projection OCT fundus images show features such as the nerve fiber layer, retinal capillary networks and choroidal vasculature. Doppler OCT images and quantitative measurements show pulsatility in retinal blood vessels. Doppler OCT provides non-invasive in vivo quantitative measurements of retinal blood flow properties and may benefit studies of diseases such as glaucoma and diabetic retinopathy. Ultrahigh speed imaging using ultrahigh resolution spectral / Fourier domain OCT promises to enable novel protocols for measuring small animal retinal structure and retinal blood flow. This non-invasive imaging technology is a promising tool for monitoring disease progression in rat and mouse models to assess ocular disease pathogenesis and response to treatment.

Original languageEnglish (US)
Title of host publicationOphthalmic Technologies XX
Volume7550
DOIs
StatePublished - Dec 1 2010
Externally publishedYes
EventOphthalmic Technologies XX - San Francisco, CA, United States
Duration: Jan 23 2010Jan 25 2010

Other

OtherOphthalmic Technologies XX
CountryUnited States
CitySan Francisco, CA
Period1/23/101/25/10

Fingerprint

retina
Spectral resolution
spectral resolution
rats
Retina
Rats
Imaging techniques
Artifacts
Blood
blood flow
Animal Structures
artifacts
Retinal Vessels
Eye Diseases
Blood vessels
glaucoma
Diabetic Retinopathy
Nerve Fibers
nerve fibers
Glaucoma

Keywords

  • Doppler OCT
  • Small animal imaging
  • Spectral/Fourier domain OCT
  • Ultrahigh resolution OCT
  • Ultrahigh speed OCT

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Liu, J. J., Potsaid, B., Chen, Y., Gorczynska, I., Srinivasan, V., Duker, J. S., & Fujimoto, J. G. (2010). Ultrahigh speed imaging of the rat retina using ultrahigh resolution spectral / Fourier domain OCT. In Ophthalmic Technologies XX (Vol. 7550). [755017] https://doi.org/10.1117/12.842540

Ultrahigh speed imaging of the rat retina using ultrahigh resolution spectral / Fourier domain OCT. / Liu, Jonathan J.; Potsaid, Benjamin; Chen, Yueli; Gorczynska, Iwona; Srinivasan, Vivek; Duker, Jay S.; Fujimoto, James G.

Ophthalmic Technologies XX. Vol. 7550 2010. 755017.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Liu, JJ, Potsaid, B, Chen, Y, Gorczynska, I, Srinivasan, V, Duker, JS & Fujimoto, JG 2010, Ultrahigh speed imaging of the rat retina using ultrahigh resolution spectral / Fourier domain OCT. in Ophthalmic Technologies XX. vol. 7550, 755017, Ophthalmic Technologies XX, San Francisco, CA, United States, 1/23/10. https://doi.org/10.1117/12.842540
Liu JJ, Potsaid B, Chen Y, Gorczynska I, Srinivasan V, Duker JS et al. Ultrahigh speed imaging of the rat retina using ultrahigh resolution spectral / Fourier domain OCT. In Ophthalmic Technologies XX. Vol. 7550. 2010. 755017 https://doi.org/10.1117/12.842540
Liu, Jonathan J. ; Potsaid, Benjamin ; Chen, Yueli ; Gorczynska, Iwona ; Srinivasan, Vivek ; Duker, Jay S. ; Fujimoto, James G. / Ultrahigh speed imaging of the rat retina using ultrahigh resolution spectral / Fourier domain OCT. Ophthalmic Technologies XX. Vol. 7550 2010.
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