Ultrahigh speed imaging of the rat retina using ultrahigh resolution spectral / Fourier domain OCT

Jonathan J. Liu, Benjamin Potsaid, Yueli Chen, Iwona Gorczynska, Vivek Srinivasan, Jay S. Duker, James G. Fujimoto

Research output: Chapter in Book/Report/Conference proceedingConference contribution


We performed OCT imaging of the rat retina at 70,000 axial scans per second with ∼3 μm axial resolution. Three-dimensional OCT (3D-OCT) data sets of the rat retina were acquired. The high speed and high density data sets enable improved en face visualization by reducing eye motion artifacts and improve Doppler OCT measurements. Minimal motion artifacts were visible and the OCT fundus images offer more precise registration of individual OCT images to retinal fundus features. Projection OCT fundus images show features such as the nerve fiber layer, retinal capillary networks and choroidal vasculature. Doppler OCT images and quantitative measurements show pulsatility in retinal blood vessels. Doppler OCT provides non-invasive in vivo quantitative measurements of retinal blood flow properties and may benefit studies of diseases such as glaucoma and diabetic retinopathy. Ultrahigh speed imaging using ultrahigh resolution spectral / Fourier domain OCT promises to enable novel protocols for measuring small animal retinal structure and retinal blood flow. This non-invasive imaging technology is a promising tool for monitoring disease progression in rat and mouse models to assess ocular disease pathogenesis and response to treatment.

Original languageEnglish (US)
Title of host publicationOphthalmic Technologies XX
StatePublished - Dec 1 2010
Externally publishedYes
EventOphthalmic Technologies XX - San Francisco, CA, United States
Duration: Jan 23 2010Jan 25 2010


OtherOphthalmic Technologies XX
Country/TerritoryUnited States
CitySan Francisco, CA


  • Doppler OCT
  • Small animal imaging
  • Spectral/Fourier domain OCT
  • Ultrahigh resolution OCT
  • Ultrahigh speed OCT

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging


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