Ultrafast method for the analysis of fluorescence lifetime imaging microscopy data based on the Laguerre expansion technique

Javier A. Jo, Qiyin Fang, Laura Marcu

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

We report a new deconvolution method for fluorescence lifetime imaging microscopy (FLIM) based on the Laguerre expansion technique. The performance of this method was tested on synthetic and real FLIM images. The following interesting properties of this technique were demonstrated. 1) The fluorescence intensity decay can be estimated simultaneously for all pixels, without a priori assumption of the decay functional form. 2) The computation speed is extremely fast, performing at least two orders of magnitude faster than current algorithms. 3) The estimated maps of Laguerre expansion coefficients provide a new domain for representing FLIM information. 4) The number of images required for the analysis is relatively small, allowing reduction of the acquisition time. These findings indicate that the developed Laguerre expansion technique for FLIM analysis represents a robust and extremely fast deconvolution method that enables practical applications of FLIM in medicine, biology, biochemistry, and chemistry.

Original languageEnglish (US)
Pages (from-to)835-845
Number of pages11
JournalIEEE Journal on Selected Topics in Quantum Electronics
Volume11
Issue number4
DOIs
StatePublished - Jul 2005

Keywords

  • Discrete Laguerre basis expansion
  • Fluorescence decay deconvolution method
  • Fluorescence lifetime imaging microscopy (FLIM)
  • Global analysis

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Atomic and Molecular Physics, and Optics

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