Ultra-high density, transcript-based genetic maps of pepper define recombination in the genome and synteny among related species

Theresa Hill, Hamid Ashrafi, Sebastian Reyes Chin-Wo, Kevin Stoffel, Maria Jose Truco, Alexander Kozik, Richard W Michelmore, Allen Van Deynze

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Our ability to assemble complex genomes and construct ultradense genetic maps now allows the determination of recombination rates, translocations, and the extent of genomic collinearity between populations, species, and genera. We developed two ultradense genetic linkage maps for pepper from single-position polymorphisms (SPPs) identified de novo with a 30,173 unigene pepper genotyping array. The Capsicum frutescens × C. annuum interspecific and the C. annuum intraspecific genetic maps were constructed comprising 16,167 and 3,878 unigene markers in 2108 and 783 genetic bins, respectively. Accuracies of marker groupings and orders are validated by the high degree of collinearity between the two maps. Marker density was sufficient to locate the chromosomal breakpoint resulting in the P1/P8 translocation between C. frutescens and C. annuum to a single bin. The two maps aligned to the pepper genome showed varying marker density along the chromosomes. There were extensive chromosomal regions with suppressed recombination and reduced intraspecific marker density. These regions corresponded to the pronounced nonrecombining pericentromeric regions in tomato, a related Solanaceous species. Similar to tomato, the extent of reduced recombination appears to be more pronounced in pepper than in other plant species. Alignment of maps with the tomato and potato genomes shows the presence of previously known translocations and a translocation event that was not observed in previous genetic maps of pepper.

Original languageEnglish (US)
Pages (from-to)2341-2355
Number of pages15
JournalG3: Genes, Genomes, Genetics
Volume5
Issue number11
DOIs
StatePublished - 2015

Fingerprint

Synteny
Lycopersicon esculentum
Genetic Recombination
Genome
Capsicum
Genetic Linkage
Solanum tuberosum
Chromosomes
Population

Keywords

  • GeneChip
  • Pseudolinkage
  • Solanum lycopersicum Solanaceae genomics
  • Solanum tuberosum

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Genetics(clinical)

Cite this

Ultra-high density, transcript-based genetic maps of pepper define recombination in the genome and synteny among related species. / Hill, Theresa; Ashrafi, Hamid; Chin-Wo, Sebastian Reyes; Stoffel, Kevin; Truco, Maria Jose; Kozik, Alexander; Michelmore, Richard W; Van Deynze, Allen.

In: G3: Genes, Genomes, Genetics, Vol. 5, No. 11, 2015, p. 2341-2355.

Research output: Contribution to journalArticle

Hill, T, Ashrafi, H, Chin-Wo, SR, Stoffel, K, Truco, MJ, Kozik, A, Michelmore, RW & Van Deynze, A 2015, 'Ultra-high density, transcript-based genetic maps of pepper define recombination in the genome and synteny among related species', G3: Genes, Genomes, Genetics, vol. 5, no. 11, pp. 2341-2355. https://doi.org/10.1534/g3.115.020040
Hill, Theresa ; Ashrafi, Hamid ; Chin-Wo, Sebastian Reyes ; Stoffel, Kevin ; Truco, Maria Jose ; Kozik, Alexander ; Michelmore, Richard W ; Van Deynze, Allen. / Ultra-high density, transcript-based genetic maps of pepper define recombination in the genome and synteny among related species. In: G3: Genes, Genomes, Genetics. 2015 ; Vol. 5, No. 11. pp. 2341-2355.
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