Type XXVII collagen at the transition of cartilage to bone during skeletogenesis

Rebecca Hjorten, Uwe Hansen, Robert A. Underwood, Helena E. Telfer, Russell J. Fernandes, Deborah Krakow, Eiman Sebald, Sebastian Wachsmann-Hogiu, Peter Bruckner, Robin Jacquet, William J. Landis, Peter H. Byers, James M. Pace

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

COL27A1 is a member of the collagen fibrillar gene family and is expressed in cartilaginous tissues including the anlage of endochondral bone. To begin to understand its role in skeletogenesis, the temporospatial distributions of its RNA message and protein product, type XXVII collagen, were determined in developing human skeletal tissues. Laser capture microdissection and quantitative reverse-transcription polymerase chain reaction demonstrated that gene expression occurred throughout the growth plate and that it was higher in the resting and proliferative zones than in hypertrophic cartilage. Immunohistochemical analyses showed that type XXVII collagen was most evident in hypertrophic cartilage at the primary ossification center and at the growth plate and that it accumulated in the pericellular matrix. Synthesis of type XXVII collagen overlapped partly with that of type X collagen, a marker of chondrocyte hypertrophy, preceded the transition of cartilage to bone, and was associated with cartilage calcification. Immunogold electron microscopy of extracted ECM components from mouse growth plate showed that type XXVII collagen was a component of long non-banded fibrous structures, filamentous networks, and thin banded fibrils. The timing and location of synthesis suggest that type XXVII collagen plays a role during the calcification of cartilage and the transition of cartilage to bone.

Original languageEnglish (US)
Pages (from-to)535-542
Number of pages8
JournalBone
Volume41
Issue number4
DOIs
StatePublished - Oct 2007

Fingerprint

Cartilage
Collagen
Bone and Bones
Growth Plate
Collagen Type X
Fibrillar Collagens
Laser Capture Microdissection
Chondrocytes
Osteogenesis
Hypertrophy
Reverse Transcription
Electron Microscopy
RNA
Gene Expression
Polymerase Chain Reaction
Genes
Proteins

Keywords

  • Bone
  • Cartilage
  • Chondrocyte
  • COL27A1
  • Endochondral
  • Growth plate
  • Skeletogenesis
  • Type XXVII collagen

ASJC Scopus subject areas

  • Physiology
  • Hematology

Cite this

Hjorten, R., Hansen, U., Underwood, R. A., Telfer, H. E., Fernandes, R. J., Krakow, D., ... Pace, J. M. (2007). Type XXVII collagen at the transition of cartilage to bone during skeletogenesis. Bone, 41(4), 535-542. https://doi.org/10.1016/j.bone.2007.06.024

Type XXVII collagen at the transition of cartilage to bone during skeletogenesis. / Hjorten, Rebecca; Hansen, Uwe; Underwood, Robert A.; Telfer, Helena E.; Fernandes, Russell J.; Krakow, Deborah; Sebald, Eiman; Wachsmann-Hogiu, Sebastian; Bruckner, Peter; Jacquet, Robin; Landis, William J.; Byers, Peter H.; Pace, James M.

In: Bone, Vol. 41, No. 4, 10.2007, p. 535-542.

Research output: Contribution to journalArticle

Hjorten, R, Hansen, U, Underwood, RA, Telfer, HE, Fernandes, RJ, Krakow, D, Sebald, E, Wachsmann-Hogiu, S, Bruckner, P, Jacquet, R, Landis, WJ, Byers, PH & Pace, JM 2007, 'Type XXVII collagen at the transition of cartilage to bone during skeletogenesis', Bone, vol. 41, no. 4, pp. 535-542. https://doi.org/10.1016/j.bone.2007.06.024
Hjorten R, Hansen U, Underwood RA, Telfer HE, Fernandes RJ, Krakow D et al. Type XXVII collagen at the transition of cartilage to bone during skeletogenesis. Bone. 2007 Oct;41(4):535-542. https://doi.org/10.1016/j.bone.2007.06.024
Hjorten, Rebecca ; Hansen, Uwe ; Underwood, Robert A. ; Telfer, Helena E. ; Fernandes, Russell J. ; Krakow, Deborah ; Sebald, Eiman ; Wachsmann-Hogiu, Sebastian ; Bruckner, Peter ; Jacquet, Robin ; Landis, William J. ; Byers, Peter H. ; Pace, James M. / Type XXVII collagen at the transition of cartilage to bone during skeletogenesis. In: Bone. 2007 ; Vol. 41, No. 4. pp. 535-542.
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