Type-I hypersensitivity as a component of eosinophilic myositis (muscular sarcocystosis) in cattle.

D. E. Granstrom, R. K. Ridley, Y. Baoan, Laurel J Gershwin, P. M. Nesbitt, L. A. Wempe

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Abstract

Eight bovine hearts with lesions of eosinophilic myositis (EM) and 2 bovine hearts without EM lesions were collected at slaughter. Blood samples from these 10 hearts, and the heart of a newborn calf also were collected. Histologically, Sarcocystis cruzi was identified in the 8 hearts with EM lesions and the 2 hearts without EM lesions, but not in the heart of the newborn calf. Serum was harvested from the 10 blood samples and was used in homologous, modified, passive cutaneous anaphylaxis tests. Antigen was prepared from S cruzi bradyzoites isolated from the 2 hearts without EM lesions. Serum samples from the 8 cattle with EM lesions reacted positively to S cruzi antigen. When heat-inactivated IgE in serum (56 C for 4 hours) was used, all passive cutaneous anaphylaxis responses were considered negative. Using ELISA, serum IgE concentrations from the 10 cattle with and without EM lesions were 2.2 to 9 U/ml. As determined by radial immunodiffusion, IgM concentrations were 80 to 215 mg/dl. Immunoglobulin G concentrations were 420 to 2,050 mg/dl, but most were less than or equal to 1,700 mg/dl. Immunoglobulin A concentrations were 0 to 62 mg/dl; 1 steer with EM lesions had 0 mg/dl. Double-gel immunodiffusion confirmed the presence of Sarcocystis-specific precipitating antibodies. Sera from the 10 cattle with and without EM lesions formed at least 1 precipitin band.

Original languageEnglish (US)
Pages (from-to)571-574
Number of pages4
JournalAmerican Journal of Veterinary Research
Volume50
Issue number4
StatePublished - Apr 1989
Externally publishedYes

Fingerprint

Sarcocystosis
sarcocystosis
immediate hypersensitivity
myositis
Immediate Hypersensitivity
lesions (animal)
heart
blood serum
cattle
Sarcocystis
anaphylaxis
Serum
Passive Cutaneous Anaphylaxis
Immunodiffusion
Immunoglobulin E
neonates
Sarcocystis cruzi
calves
bradyzoites
antigens

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Type-I hypersensitivity as a component of eosinophilic myositis (muscular sarcocystosis) in cattle. / Granstrom, D. E.; Ridley, R. K.; Baoan, Y.; Gershwin, Laurel J; Nesbitt, P. M.; Wempe, L. A.

In: American Journal of Veterinary Research, Vol. 50, No. 4, 04.1989, p. 571-574.

Research output: Contribution to journalArticle

Granstrom, D. E. ; Ridley, R. K. ; Baoan, Y. ; Gershwin, Laurel J ; Nesbitt, P. M. ; Wempe, L. A. / Type-I hypersensitivity as a component of eosinophilic myositis (muscular sarcocystosis) in cattle. In: American Journal of Veterinary Research. 1989 ; Vol. 50, No. 4. pp. 571-574.
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