Two‐dimensional electrophoresis of the plasma proteins of alpacas and llamas: genetic polymorphism of α1B‐glycoprotein and three other proteins

R. K. JUNEJA, Cecilia Penedo, H. E.B. LARSSON, B. GAHNE, A. T. BOWLING

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Summary. Plasma samples of alpacas and llamas were analysed by a simple method of two‐dimensional (2‐D) agarose gel (pH 8.6)‐horizontal polyacrylamide gel (pH 9.0) electrophoresis, followed by general protein staining of gels. Genetic polymorphism in both species is described for α1B‐glycoprotein (α1B) and three other unidentified proteins designated prealbumin (Pr), postalbumin 1 and 2 (Pal and Pa2). α1B was identified by cross‐reactivity with antisera for human and pig α1B. Altogether, two alleles of Pr, two of Pa1, five of α1B and three of Pa2 are described. Most of the alleles were present in alpacas and llamas. Alpacas showed a high degree of polymorphism at all four loci. Llamas showed considerable polymorphism at only the Pa1 and Pa2 loci. The theoretical probability of exclusion (Pe) of an incorrectly assigned parent was estimated to be about 80% in each species by typing for the six polymorphic plasma proteins reported so far in these species. The given method of 2‐D electrophoresis revealed no fixed differences in protein mobilities that discriminate between llamas and alpacas.

Original languageEnglish (US)
Pages (from-to)395-406
Number of pages12
JournalAnimal Genetics
Volume20
DOIs
StatePublished - Jan 1 1989

Fingerprint

New World Camelids
llamas
alpacas
Genetic Polymorphisms
blood proteins
electrophoresis
Electrophoresis
Blood Proteins
genetic polymorphism
prealbumin
gels
Proteins
proteins
alleles
loci
Prealbumin
polyacrylamide
agarose
antiserum
Gels

Keywords

  • alpaca
  • camelids
  • genetic polymorphism
  • immunoblotting
  • llama
  • plasma proteins
  • α1B‐glycoprotein

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Genetics

Cite this

Two‐dimensional electrophoresis of the plasma proteins of alpacas and llamas : genetic polymorphism of α1B‐glycoprotein and three other proteins. / JUNEJA, R. K.; Penedo, Cecilia; LARSSON, H. E.B.; GAHNE, B.; BOWLING, A. T.

In: Animal Genetics, Vol. 20, 01.01.1989, p. 395-406.

Research output: Contribution to journalArticle

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