Two-tiered electrical detection, purification, and identification of nucleic acids in complex media

Jovana Veselinovic, Mashari Alangari, Yuanhui Li, Zimple Matharu, Juan M. Artés, Erkin Seker, Joshua Hihath

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We demonstrate a two-tiered platform for electrically detecting, purifying, and distinguishing mismatched nucleic acid sequences (DNA and RNA)in complex biological liquid samples. The first tier uses a nanoporous gold (np-Au)for the electrochemical detection and subsequent purification of nucleic acids. The sieve-like structure of np-Au blocks the permeation of large biomolecules and allows for selective transport of nucleic acids to the sensing surface, thereby obviating the need for prior purification steps. The second tier utilizes single-molecule electrical conductance measurements to verify sequence information and identify the presence of mismatches between the DNA probe and the RNA target. This step utilizes the single-molecule break junction approach to measure samples eluted from the first-tier to reduce the possibility of false positives from similar target sequences. In this report, the two-tiered approach is employed to detect a 15-base pair (bp)E. coli. RNA sequence in blood, and is further expanded to differentiate between the perfectly-matched case and partial mismatches of the DNA:RNA hybrids.

Original languageEnglish (US)
Pages (from-to)116-121
Number of pages6
JournalElectrochimica Acta
Volume313
DOIs
StatePublished - Aug 1 2019

Fingerprint

Nucleic acids
RNA
Nucleic Acids
Purification
DNA
Nucleic acid sequences
Molecules
Sieves
DNA Probes
Biomolecules
Permeation
Gold
Escherichia coli
Blood
Liquids

Keywords

  • DNA Detection
  • Electrochemical Sensors
  • Molecular Electronics
  • Nanoporous Gold
  • Single-Molecule

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Electrochemistry

Cite this

Two-tiered electrical detection, purification, and identification of nucleic acids in complex media. / Veselinovic, Jovana; Alangari, Mashari; Li, Yuanhui; Matharu, Zimple; Artés, Juan M.; Seker, Erkin; Hihath, Joshua.

In: Electrochimica Acta, Vol. 313, 01.08.2019, p. 116-121.

Research output: Contribution to journalArticle

Veselinovic, Jovana ; Alangari, Mashari ; Li, Yuanhui ; Matharu, Zimple ; Artés, Juan M. ; Seker, Erkin ; Hihath, Joshua. / Two-tiered electrical detection, purification, and identification of nucleic acids in complex media. In: Electrochimica Acta. 2019 ; Vol. 313. pp. 116-121.
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