Two-photon microscopy of oxygen distributions in mouse cerebral microvasculature

Sava Sakadžić, Emiri T. Mandeville, Louis Gagnon, Anna Devor, Mohammad A. Yaseen, Joe Musacchia, Emmanuel Roussakis, Katharina Eikermann-Haerter, Vivek Srinivasan, Cenk Ayata, Eng H. Lo, Anders M. Dale, Sergei A. Vinogradov, David A. Boas

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Microscopic measurements of oxygen delivery and metabolism are essential for better understanding of cerebrovascular pathologies and interpretation of macroscopic data from imaging modalities such as functional magnetic resonance imaging (fMRI) and diffuse optical tomography. We developed a method to obtain detailed statistics of oxygen content in cortical microvascular networks of anesthetized mice based on two-photon microscopy phosphorescence lifetime imaging of oxygen sensitive dyes. We provide the oxygen intravascular distribution as a function of various morphological parameters such as branching order from pial arteries and veins, as well as the difference in the intravascular oxygen distribution in normocapnic and hypercapnic states.

Original languageEnglish (US)
Title of host publicationBiomedical Optics, BIOMED 2012
StatePublished - Dec 1 2012
Externally publishedYes
EventBiomedical Optics, BIOMED 2012 - Miami, FL, United States
Duration: Apr 28 2012May 2 2012

Other

OtherBiomedical Optics, BIOMED 2012
CountryUnited States
CityMiami, FL
Period4/28/125/2/12

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ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

Cite this

Sakadžić, S., Mandeville, E. T., Gagnon, L., Devor, A., Yaseen, M. A., Musacchia, J., Roussakis, E., Eikermann-Haerter, K., Srinivasan, V., Ayata, C., Lo, E. H., Dale, A. M., Vinogradov, S. A., & Boas, D. A. (2012). Two-photon microscopy of oxygen distributions in mouse cerebral microvasculature. In Biomedical Optics, BIOMED 2012