Two-photon microscopy of oxygen distributions in mouse cerebral microvasculature

Sava Sakadžić; Emiri T. Mandeville; Louis Gagnon; Anna Devor; Mohammad A. Yaseen; Joe Musacchia; Emmanuel Roussakis; Katharina Eikermann-Haerter; Vivek Srinivasan; Cenk Ayata; Eng H. Lo; Anders M. Dale; Sergei A. Vinogradov; David A. Boas

Two-photon microscopy of oxygen distributions in mouse cerebral microvasculature

Sava Sakadžić, Emiri T. Mandeville, Louis Gagnon, Anna Devor, Mohammad A. Yaseen, Joe Musacchia, Emmanuel Roussakis, Katharina Eikermann-Haerter, Vivek Srinivasan, Cenk Ayata, Eng H. Lo, Anders M. Dale, Sergei A. Vinogradov, David A. Boas

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Microscopic measurements of oxygen delivery and metabolism are essential for better understanding of cerebrovascular pathologies and interpretation of macroscopic data from imaging modalities such as functional magnetic resonance imaging (fMRI) and diffuse optical tomography. We developed a method to obtain detailed statistics of oxygen content in cortical microvascular networks of anesthetized mice based on two-photon microscopy phosphorescence lifetime imaging of oxygen sensitive dyes. We provide the oxygen intravascular distribution as a function of various morphological parameters such as branching order from pial arteries and veins, as well as the difference in the intravascular oxygen distribution in normocapnic and hypercapnic states.

Original language	English (US)
Title of host publication	Biomedical Optics, BIOMED 2012
State	Published - Dec 1 2012
Externally published	Yes
Event	Biomedical Optics, BIOMED 2012 - Miami, FL, United States Duration: Apr 28 2012 → May 2 2012

Other

Other	Biomedical Optics, BIOMED 2012
Country	United States
City	Miami, FL
Period	4/28/12 → 5/2/12

ASJC Scopus subject areas

Biotechnology
Atomic and Molecular Physics, and Optics

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Two-photon microscopy of oxygen distributions in mouse cerebral microvasculature. / Sakadžić, Sava; Mandeville, Emiri T.; Gagnon, Louis; Devor, Anna; Yaseen, Mohammad A.; Musacchia, Joe; Roussakis, Emmanuel; Eikermann-Haerter, Katharina; Srinivasan, Vivek; Ayata, Cenk; Lo, Eng H.; Dale, Anders M.; Vinogradov, Sergei A.; Boas, David A.

Biomedical Optics, BIOMED 2012. 2012.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Sakadžić, S, Mandeville, ET, Gagnon, L, Devor, A, Yaseen, MA, Musacchia, J, Roussakis, E, Eikermann-Haerter, K, Srinivasan, V, Ayata, C, Lo, EH, Dale, AM, Vinogradov, SA & Boas, DA 2012, Two-photon microscopy of oxygen distributions in mouse cerebral microvasculature. in Biomedical Optics, BIOMED 2012. Biomedical Optics, BIOMED 2012, Miami, FL, United States, 4/28/12.

Sakadžić, Sava ; Mandeville, Emiri T. ; Gagnon, Louis ; Devor, Anna ; Yaseen, Mohammad A. ; Musacchia, Joe ; Roussakis, Emmanuel ; Eikermann-Haerter, Katharina ; Srinivasan, Vivek ; Ayata, Cenk ; Lo, Eng H. ; Dale, Anders M. ; Vinogradov, Sergei A. ; Boas, David A. / Two-photon microscopy of oxygen distributions in mouse cerebral microvasculature. Biomedical Optics, BIOMED 2012. 2012.

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AU - Musacchia, Joe

AU - Roussakis, Emmanuel

AU - Eikermann-Haerter, Katharina

AU - Srinivasan, Vivek

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AU - Lo, Eng H.

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AU - Vinogradov, Sergei A.

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