Abstract
Microscopic measurements of oxygen delivery and metabolism are essential for better understanding of cerebrovascular pathologies and interpretation of macroscopic data from imaging modalities such as functional magnetic resonance imaging (fMRI) and diffuse optical tomography. We developed a method to obtain detailed statistics of oxygen content in cortical microvascular networks of anesthetized mice based on two-photon microscopy phosphorescence lifetime imaging of oxygen sensitive dyes. We provide the oxygen intravascular distribution as a function of various morphological parameters such as branching order from pial arteries and veins, as well as the difference in the intravascular oxygen distribution in normocapnic and hypercapnic states.
| Original language | English (US) |
|---|---|
| Title of host publication | Biomedical Optics, BIOMED 2012 |
| State | Published - Dec 1 2012 |
| Externally published | Yes |
| Event | Biomedical Optics, BIOMED 2012 - Miami, FL, United States Duration: Apr 28 2012 → May 2 2012 |
Other
| Other | Biomedical Optics, BIOMED 2012 |
|---|---|
| Country | United States |
| City | Miami, FL |
| Period | 4/28/12 → 5/2/12 |
Fingerprint
ASJC Scopus subject areas
- Biotechnology
- Atomic and Molecular Physics, and Optics
Cite this
Two-photon microscopy of oxygen distributions in mouse cerebral microvasculature. / Sakadžić, Sava; Mandeville, Emiri T.; Gagnon, Louis; Devor, Anna; Yaseen, Mohammad A.; Musacchia, Joe; Roussakis, Emmanuel; Eikermann-Haerter, Katharina; Srinivasan, Vivek; Ayata, Cenk; Lo, Eng H.; Dale, Anders M.; Vinogradov, Sergei A.; Boas, David A.
Biomedical Optics, BIOMED 2012. 2012.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Two-photon microscopy of oxygen distributions in mouse cerebral microvasculature
AU - Sakadžić, Sava
AU - Mandeville, Emiri T.
AU - Gagnon, Louis
AU - Devor, Anna
AU - Yaseen, Mohammad A.
AU - Musacchia, Joe
AU - Roussakis, Emmanuel
AU - Eikermann-Haerter, Katharina
AU - Srinivasan, Vivek
AU - Ayata, Cenk
AU - Lo, Eng H.
AU - Dale, Anders M.
AU - Vinogradov, Sergei A.
AU - Boas, David A.
PY - 2012/12/1
Y1 - 2012/12/1
N2 - Microscopic measurements of oxygen delivery and metabolism are essential for better understanding of cerebrovascular pathologies and interpretation of macroscopic data from imaging modalities such as functional magnetic resonance imaging (fMRI) and diffuse optical tomography. We developed a method to obtain detailed statistics of oxygen content in cortical microvascular networks of anesthetized mice based on two-photon microscopy phosphorescence lifetime imaging of oxygen sensitive dyes. We provide the oxygen intravascular distribution as a function of various morphological parameters such as branching order from pial arteries and veins, as well as the difference in the intravascular oxygen distribution in normocapnic and hypercapnic states.
AB - Microscopic measurements of oxygen delivery and metabolism are essential for better understanding of cerebrovascular pathologies and interpretation of macroscopic data from imaging modalities such as functional magnetic resonance imaging (fMRI) and diffuse optical tomography. We developed a method to obtain detailed statistics of oxygen content in cortical microvascular networks of anesthetized mice based on two-photon microscopy phosphorescence lifetime imaging of oxygen sensitive dyes. We provide the oxygen intravascular distribution as a function of various morphological parameters such as branching order from pial arteries and veins, as well as the difference in the intravascular oxygen distribution in normocapnic and hypercapnic states.
UR - http://www.scopus.com/inward/record.url?scp=84890677903&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84890677903&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84890677903
SN - 9781557529428
BT - Biomedical Optics, BIOMED 2012
ER -