Two-photon microscopy of oxygen distributions in mouse cerebral microvasculature

Sava Sakadžić, Emiri T. Mandeville, Louis Gagnon, Anna Devor, Mohammad A. Yaseen, Joe Musacchia, Emmanuel Roussakis, Katharina Eikermann-Haerter, Vivek Srinivasan, Cenk Ayata, Eng H. Lo, Anders M. Dale, Sergei A. Vinogradov, David A. Boas

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Microscopic measurements of oxygen delivery and metabolism are essential for better understanding of cerebrovascular pathologies and interpretation of macroscopic data from imaging modalities such as functional magnetic resonance imaging (fMRI) and diffuse optical tomography. We developed a method to obtain detailed statistics of oxygen content in cortical microvascular networks of anesthetized mice based on two-photon microscopy phosphorescence lifetime imaging of oxygen sensitive dyes. We provide the oxygen intravascular distribution as a function of various morphological parameters such as branching order from pial arteries and veins, as well as the difference in the intravascular oxygen distribution in normocapnic and hypercapnic states.

Original languageEnglish (US)
Title of host publicationBiomedical Optics, BIOMED 2012
StatePublished - Dec 1 2012
Externally publishedYes
EventBiomedical Optics, BIOMED 2012 - Miami, FL, United States
Duration: Apr 28 2012May 2 2012

Other

OtherBiomedical Optics, BIOMED 2012
CountryUnited States
CityMiami, FL
Period4/28/125/2/12

Fingerprint

Microvessels
Photons
mice
Microscopy
Oxygen
microscopy
photons
oxygen
Optical Tomography
Cerebral Veins
pathology
metabolism
phosphorescence
arteries
veins
magnetic resonance
delivery
Coloring Agents
Arteries
tomography

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

Cite this

Sakadžić, S., Mandeville, E. T., Gagnon, L., Devor, A., Yaseen, M. A., Musacchia, J., ... Boas, D. A. (2012). Two-photon microscopy of oxygen distributions in mouse cerebral microvasculature. In Biomedical Optics, BIOMED 2012

Two-photon microscopy of oxygen distributions in mouse cerebral microvasculature. / Sakadžić, Sava; Mandeville, Emiri T.; Gagnon, Louis; Devor, Anna; Yaseen, Mohammad A.; Musacchia, Joe; Roussakis, Emmanuel; Eikermann-Haerter, Katharina; Srinivasan, Vivek; Ayata, Cenk; Lo, Eng H.; Dale, Anders M.; Vinogradov, Sergei A.; Boas, David A.

Biomedical Optics, BIOMED 2012. 2012.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Sakadžić, S, Mandeville, ET, Gagnon, L, Devor, A, Yaseen, MA, Musacchia, J, Roussakis, E, Eikermann-Haerter, K, Srinivasan, V, Ayata, C, Lo, EH, Dale, AM, Vinogradov, SA & Boas, DA 2012, Two-photon microscopy of oxygen distributions in mouse cerebral microvasculature. in Biomedical Optics, BIOMED 2012. Biomedical Optics, BIOMED 2012, Miami, FL, United States, 4/28/12.
Sakadžić S, Mandeville ET, Gagnon L, Devor A, Yaseen MA, Musacchia J et al. Two-photon microscopy of oxygen distributions in mouse cerebral microvasculature. In Biomedical Optics, BIOMED 2012. 2012
Sakadžić, Sava ; Mandeville, Emiri T. ; Gagnon, Louis ; Devor, Anna ; Yaseen, Mohammad A. ; Musacchia, Joe ; Roussakis, Emmanuel ; Eikermann-Haerter, Katharina ; Srinivasan, Vivek ; Ayata, Cenk ; Lo, Eng H. ; Dale, Anders M. ; Vinogradov, Sergei A. ; Boas, David A. / Two-photon microscopy of oxygen distributions in mouse cerebral microvasculature. Biomedical Optics, BIOMED 2012. 2012.
@inproceedings{9c13cf27894a494592fb5c57f08f4b0c,
title = "Two-photon microscopy of oxygen distributions in mouse cerebral microvasculature",
abstract = "Microscopic measurements of oxygen delivery and metabolism are essential for better understanding of cerebrovascular pathologies and interpretation of macroscopic data from imaging modalities such as functional magnetic resonance imaging (fMRI) and diffuse optical tomography. We developed a method to obtain detailed statistics of oxygen content in cortical microvascular networks of anesthetized mice based on two-photon microscopy phosphorescence lifetime imaging of oxygen sensitive dyes. We provide the oxygen intravascular distribution as a function of various morphological parameters such as branching order from pial arteries and veins, as well as the difference in the intravascular oxygen distribution in normocapnic and hypercapnic states.",
author = "Sava Sakadžić and Mandeville, {Emiri T.} and Louis Gagnon and Anna Devor and Yaseen, {Mohammad A.} and Joe Musacchia and Emmanuel Roussakis and Katharina Eikermann-Haerter and Vivek Srinivasan and Cenk Ayata and Lo, {Eng H.} and Dale, {Anders M.} and Vinogradov, {Sergei A.} and Boas, {David A.}",
year = "2012",
month = "12",
day = "1",
language = "English (US)",
isbn = "9781557529428",
booktitle = "Biomedical Optics, BIOMED 2012",

}

TY - GEN

T1 - Two-photon microscopy of oxygen distributions in mouse cerebral microvasculature

AU - Sakadžić, Sava

AU - Mandeville, Emiri T.

AU - Gagnon, Louis

AU - Devor, Anna

AU - Yaseen, Mohammad A.

AU - Musacchia, Joe

AU - Roussakis, Emmanuel

AU - Eikermann-Haerter, Katharina

AU - Srinivasan, Vivek

AU - Ayata, Cenk

AU - Lo, Eng H.

AU - Dale, Anders M.

AU - Vinogradov, Sergei A.

AU - Boas, David A.

PY - 2012/12/1

Y1 - 2012/12/1

N2 - Microscopic measurements of oxygen delivery and metabolism are essential for better understanding of cerebrovascular pathologies and interpretation of macroscopic data from imaging modalities such as functional magnetic resonance imaging (fMRI) and diffuse optical tomography. We developed a method to obtain detailed statistics of oxygen content in cortical microvascular networks of anesthetized mice based on two-photon microscopy phosphorescence lifetime imaging of oxygen sensitive dyes. We provide the oxygen intravascular distribution as a function of various morphological parameters such as branching order from pial arteries and veins, as well as the difference in the intravascular oxygen distribution in normocapnic and hypercapnic states.

AB - Microscopic measurements of oxygen delivery and metabolism are essential for better understanding of cerebrovascular pathologies and interpretation of macroscopic data from imaging modalities such as functional magnetic resonance imaging (fMRI) and diffuse optical tomography. We developed a method to obtain detailed statistics of oxygen content in cortical microvascular networks of anesthetized mice based on two-photon microscopy phosphorescence lifetime imaging of oxygen sensitive dyes. We provide the oxygen intravascular distribution as a function of various morphological parameters such as branching order from pial arteries and veins, as well as the difference in the intravascular oxygen distribution in normocapnic and hypercapnic states.

UR - http://www.scopus.com/inward/record.url?scp=84890677903&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84890677903&partnerID=8YFLogxK

M3 - Conference contribution

SN - 9781557529428

BT - Biomedical Optics, BIOMED 2012

ER -