Two mechanisms of quantized calcium release in skeletal muscle

M. G. Klein, H. Cheng, Luis Fernando Santana, Y. H. Jiang, W. J. Lederer, M. F. Schneider

Research output: Contribution to journalArticle

267 Scopus citations

Abstract

SKELETAL muscle uses voltage sensors in the transverse tubular membrane that are linked by protein-protein interactions to intracellular ryanodine receptors, which gate the release of calcium from the sarcoplasmic reticulum. Here we show, by using voltage-clamped single fibres and confocal imaging, that stochastic calcium-release events, visualized as Ca2+ sparks, occur in skeletal muscle and originate at the triad. Unitary triadic Ca2+ release events are initiated by the voltage sensor in a steeply voltage-dependent manner, or occur spontaneously by a mechanism independent of the voltage sensor. Large amplitude events also occur during depolarization and consist of two or more unitary events. We propose a 'dual control' model for discrete Ca2+ release events from the sacroplasmic reticulum that unifies diverse observations about Ca2+-signalling in frog skeletal muscle, and that may be applicable to other excitable cells.

Original languageEnglish (US)
Pages (from-to)455-458
Number of pages4
JournalNature
Volume379
Issue number6564
DOIs
StatePublished - Feb 1 1996
Externally publishedYes

ASJC Scopus subject areas

  • General

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    Klein, M. G., Cheng, H., Santana, L. F., Jiang, Y. H., Lederer, W. J., & Schneider, M. F. (1996). Two mechanisms of quantized calcium release in skeletal muscle. Nature, 379(6564), 455-458. https://doi.org/10.1038/379455a0