Two independent and interactive DNA-binding subdomains of the Pax6 paired domain are regulated by alternative splicing

Jonathan A. Epstein, Thomas M Glaser, Jiexing Cai, Lisa Jepeal, David S. Walton, Richard L. Maas

Research output: Contribution to journalArticle

295 Citations (Scopus)

Abstract

Vertebrate Pax proteins share a conserved 128-amino-acid DNA-binding motif, the paired domain. The PAX6 gene, which is mutated in the murine Small eye and human aniridia developmental defects, also encodes a second protein with a 14-amino-acid insertion in the paired domain. This protein, which arises by alternative mRNA splicing, exhibits unique DNA-binding properties. Unlike other paired domains, which bind DNA predominantly by their amino termini, the extended Pax6 paired domain interacts with DNA exclusively through its carboxyl terminus. This property can be simulated by deletion of 30 amino-terminal residues from the Pax6 or Pax2 paired domains. Thus, the insertion acts as a molecular toggle to unmask the DNA-binding potential of the carboxyl terminus. The functional nonequivalence of the two Pax6 proteins is underscored by a T → C mutation at position -3 of the alternative splice acceptor site that changes the ratio of the two isoforms and causes a distinct human ocular syndrome.

Original languageEnglish (US)
Pages (from-to)2022-2034
Number of pages13
JournalGenes and Development
Volume8
Issue number17
StatePublished - Sep 1 1994
Externally publishedYes

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Alternative Splicing
RNA Splice Sites
DNA
Proteins
Aniridia
Amino Acids
Nucleotide Motifs
Vertebrates
Protein Isoforms
Messenger RNA
Mutation
Genes

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Two independent and interactive DNA-binding subdomains of the Pax6 paired domain are regulated by alternative splicing. / Epstein, Jonathan A.; Glaser, Thomas M; Cai, Jiexing; Jepeal, Lisa; Walton, David S.; Maas, Richard L.

In: Genes and Development, Vol. 8, No. 17, 01.09.1994, p. 2022-2034.

Research output: Contribution to journalArticle

Epstein, Jonathan A. ; Glaser, Thomas M ; Cai, Jiexing ; Jepeal, Lisa ; Walton, David S. ; Maas, Richard L. / Two independent and interactive DNA-binding subdomains of the Pax6 paired domain are regulated by alternative splicing. In: Genes and Development. 1994 ; Vol. 8, No. 17. pp. 2022-2034.
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