Two Distinct Surveillance Mechanisms Monitor Meiotic Chromosome Metabolism in Budding Yeast

Hsin Yen Wu, Sean M. Burgess

Research output: Contribution to journalArticle

66 Scopus citations

Abstract

Meiotic recombination is initiated by Spo11-generated DNA double-strand breaks (DSBs) [1]. A fraction of total DSBs is processed into crossovers (CRs) between homologous chromosomes, which promote their accurate segregation at meiosis I (MI) [2]. The coordination of recombination-associated events and MI progression is governed by the "pachytene checkpoint" [3], which in budding yeast requires Rad17, a component of a PCNA clamp-like complex, and Pch2, a putative AAA-ATPase [3-7]. We show that two genetically separable pathways monitor the presence of distinct meiotic recombination-associated lesions: First, delayed MI progression in the presence of DNA repair intermediates is suppressed when RAD17 or SAE2, encoding a DSB-end processing factor [8, 9], is deleted. Second, delayed MI progression in the presence of aberrant synaptonemal complex (SC) is suppressed when PCH2 is deleted. Importantly, ZIP1, encoding the central element of the SC [10], is required for PCH2-dependent checkpoint activation. Analysis of the rad17Δ pch2Δ double mutant revealed a redundant function regulating interhomolog CR formation. These findings suggest a link between the surveillance of distinct recombination-associated lesions, control of CR formation kinetics, and regulation of MI timing. A PCH2-ZIP1-dependent checkpoint in meiosis is likely conserved among synaptic organisms from yeast to human [6, 11].

Original languageEnglish (US)
Pages (from-to)2473-2479
Number of pages7
JournalCurrent Biology
Volume16
Issue number24
DOIs
StatePublished - Dec 19 2006

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Keywords

  • DNA

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

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