Abstract
Francis, D. M., and Michelmore, R. W. 1993. Two classes of chromosome-sized molecules are present in Bremia lactucae. Experimental Mycology, 17, 284-300. The size and number of chromosomes from Bremia lactucae have been estimated using pulsed-field gel electrophoresis. The karyotype consists of a minimum of seven chromosomes between 3.0 and at least 8 Mb and a set of polymorphic molecules between 300 kb and 1.6 Mb. Genetic and hybridization analyses confirmed the distinction between the large chromosomes and the smaller polymorphic molecules. The polymorphic molecules are linear, nonribosomal, and located in the nucleus; they are related in sequence and do not segregate in a Mendelian fashion. The polymorphic molecules are therefore either B chromosomes or large linear plasmids. Chromosomes carrying two avirulence genes and several restriction fragment length polymorphism markers have been identified.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 284-300 |
| Number of pages | 17 |
| Journal | Experimental Mycology |
| Volume | 17 |
| Issue number | 4 |
| DOIs | |
| State | Published - Dec 1993 |
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Keywords
- avirulence genes
- B chromosomes
- Bremia lactucae
- karyotype
- Oomycete
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
Cite this
Two Classes of Chromosome-Sized Molecules Are Present in Bremia lactucae. / Francis, David M.; Michelmore, Richard W.
In: Experimental Mycology, Vol. 17, No. 4, 12.1993, p. 284-300.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Two Classes of Chromosome-Sized Molecules Are Present in Bremia lactucae
AU - Francis, David M.
AU - Michelmore, Richard W
PY - 1993/12
Y1 - 1993/12
N2 - Francis, D. M., and Michelmore, R. W. 1993. Two classes of chromosome-sized molecules are present in Bremia lactucae. Experimental Mycology, 17, 284-300. The size and number of chromosomes from Bremia lactucae have been estimated using pulsed-field gel electrophoresis. The karyotype consists of a minimum of seven chromosomes between 3.0 and at least 8 Mb and a set of polymorphic molecules between 300 kb and 1.6 Mb. Genetic and hybridization analyses confirmed the distinction between the large chromosomes and the smaller polymorphic molecules. The polymorphic molecules are linear, nonribosomal, and located in the nucleus; they are related in sequence and do not segregate in a Mendelian fashion. The polymorphic molecules are therefore either B chromosomes or large linear plasmids. Chromosomes carrying two avirulence genes and several restriction fragment length polymorphism markers have been identified.
AB - Francis, D. M., and Michelmore, R. W. 1993. Two classes of chromosome-sized molecules are present in Bremia lactucae. Experimental Mycology, 17, 284-300. The size and number of chromosomes from Bremia lactucae have been estimated using pulsed-field gel electrophoresis. The karyotype consists of a minimum of seven chromosomes between 3.0 and at least 8 Mb and a set of polymorphic molecules between 300 kb and 1.6 Mb. Genetic and hybridization analyses confirmed the distinction between the large chromosomes and the smaller polymorphic molecules. The polymorphic molecules are linear, nonribosomal, and located in the nucleus; they are related in sequence and do not segregate in a Mendelian fashion. The polymorphic molecules are therefore either B chromosomes or large linear plasmids. Chromosomes carrying two avirulence genes and several restriction fragment length polymorphism markers have been identified.
KW - avirulence genes
KW - B chromosomes
KW - Bremia lactucae
KW - karyotype
KW - Oomycete
UR - http://www.scopus.com/inward/record.url?scp=0027787569&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027787569&partnerID=8YFLogxK
U2 - 10.1006/emyc.1993.1027
DO - 10.1006/emyc.1993.1027
M3 - Article
AN - SCOPUS:0027787569
VL - 17
SP - 284
EP - 300
JO - Fungal Genetics and Biology
JF - Fungal Genetics and Biology
SN - 1087-1845
IS - 4
ER -